We narrowed to 35,158 results for: IND

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pBabe HAII RLF CAAX

Plasmid

#12592

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

RLF-CAAX (Rgl2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pEPQD0CM0068

Plasmid

#177025

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

major latex protein-like

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM28521

Plasmid

#105359

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

EC1.2en-EC1.1p fusion promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCF708

Plasmid

#121685

Purpose

EF1a-dTEV-protease-mTagBFP2

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

TEV protease

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Cenp-B DBD INCENP mCherry

Plasmid

#45233

Purpose

Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B-DBD INCENP-Δcen mCherry

Tags

DNA binding domain of CENP-B and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pet 3d alpha 1 beta 2 CP

Plasmid

#13450

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLD-hygro-EnVM

Plasmid

#24590

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VM

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
p3003 pGEM E6-AP

Plasmid

#8644

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
nGR2175-gs10-NLS-VPR

Plasmid

#188264

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nGR2175

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAL612

Plasmid

#119860

Purpose

precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine

Tags

none

Expression

Bacterial

Mutation

Cysterine 290 to Serine and Glycine 244 to Cystei…

Promoter

lac

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-10u

Plasmid

#13542

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-10u

Plasmid

#13546

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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Lyn-tdnano-HA

Plasmid

#136512

Purpose

Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

Lyn nano nano 3HA

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAID-CMV linearizing in pX330

Plasmid

#140609

Purpose

Crispr/Cas9 plasmid for linearization of pAID-CMV

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-1m

Plasmid

#13548

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA7woH

Plasmid

#65020

Purpose

Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, E105K, E108A, D16…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

Plasmid

#91934

Purpose

Bacterial expression of maize SUMO1a and SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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