2,159 results

1. Tol2-elavl3-H2B-GCaMP6s

   Plasmid

   #59530

   Purpose

   Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

   Depositor

   Misha Ahrens

   Article

   Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

   Inserts

   elavl3 promoter (elavl3 Zebrafish)
   H2B
   GCaMP6s
   

   Use

   Transposable element

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. zCREST3:redCas9; control_gRNA

   Plasmid

   #199334

   Purpose

   pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   control sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZE31 Prickle-1-pCS2P+

   Plasmid

   #17072

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   Prickle-1 (prickle1a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

4. ZP46 Prickle-2

   Plasmid

   #16896

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   Prickle-2 (prickle2b Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

5. SI10-Brevican

   Plasmid

   #46300

   Depositor

   Gavin Wright

   Article

   Crosnier et al BMC Biol. 2010 . 8():76.

   Insert

   Brevican mouse monoclonal antibody (bcan Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

6. p5E_(-3kb)nrg1 type II

   Plasmid

   #199393

   Purpose

   pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   (-3kb)nrg1 type II
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E_ngn1_LSE_C

   Plasmid

   #199395

   Purpose

   pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   LSE_C
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTone-gata2aECE-Kaede

   Plasmid

   #132976

   Purpose

   gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   Kaede
   gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
   

   Use

   Unspecified; Transposon-mediated

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p160Tol2-her4.3:switchCas9

   Plasmid

   #227680

   Purpose

   Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   loxP-dsRed-loxP-Cas9-t2A-GFP
   4xU6:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and her4.3

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2-lyzC-mito-Dendra2

    Plasmid

    #107590

    Purpose

    Expresses mitochondria localized Dendra2 in zebrafish neutrophils

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    mito-Dendra2
    

    Use

    Tags

    mitochondira localization sequence

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-krt5

    Plasmid

    #82585

    Purpose

    5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    zebrafish krt5 promoter (krt5 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. slc1a3b:myrGFP-P2A-H2AmCherry

    Plasmid

    #170208

    Purpose

    Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGFP-P2A-H2AmCherry

    Expression

    Mutation

    Promoter

    slc1a3b

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGTag-NLS-eGFP-SV40

    Plasmid

    #117811

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-TODestGFP_RNAseH1

    Plasmid

    #65784

    Purpose

    expresses GFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Zebrafish, Human)
    

    Use

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-2.3k-fabp2

    Plasmid

    #84927

    Purpose

    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector

    Depositor

    John Rawls

    Insert

    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    

    Use

    Tags

    NO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 14UAS zfPSD95:GFP

    Plasmid

    #74314

    Purpose

    A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

    Depositor

    Martin Meyer, Stephen Smith

    Article

    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

    Insert

    zebrafish PSD95
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Y599H and I793S (please see depositor comment bel…

    Promoter

    UAS

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. p3E-U6a-U6c-ndufs2 guide

    Plasmid

    #107601

    Purpose

    An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    ndufs2 guide RNA sequences
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-BiNLS-fYap

    Plasmid

    #176623

    Purpose

    expresses optofYap in zebrafish

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    Yes associated protein (yap1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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