We narrowed to 1,540 results for: SEU

1. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSL3713 (pTnsC_Pse)

   Plasmid

   #200900

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsC
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNAtet-lvaA

   Plasmid

   #106397

   Purpose

   Guide RNA plasmid targeting lvaA on BBR1-UP origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   sgRNA towards lvaA in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM251 (miniCTX1-araC-ParaBAD)

   Plasmid

   #110567

   Purpose

   Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araC-ParaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK255

   Plasmid

   #171155

   Purpose

   Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   I-SceI_sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA-gRic6T

   Plasmid

   #106401

   Purpose

   E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

   Depositor

   Sheng Yang

   Article

   Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pj23119

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMBPCas2/3

   Plasmid

   #89238

   Purpose

   Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas2/3
   

   Use

   Tags

   Strep II-MBP

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Use

   Tags

   Strep II tag on Cas1

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pStrepIHFb

   Plasmid

   #149385

   Purpose

   Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
   

   Use

   Tags

   HRV3C cleavage site and StrepII-tag with

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pgRNAkan-glpR

    Plasmid

    #169873

    Purpose

    Single guide RNA plasmid for Cas9 targeting glpR in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards glpR in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. G1088E_pLenti-CMV-mNeonGreen-2A-HygroR

    Plasmid

    #216279

    Purpose

    Green and hygromycin resistant lentiviral vector plasmid for multiplexed receptor pseudovirus experiments

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2024 May 20;20(5):e1012044.

    Insert

    mNeonGreen-2A-Hpt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR4 AvrRpm1

    Plasmid

    #209185

    Purpose

    pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AvrRpm1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR2_wt

    Plasmid

    #149386

    Purpose

    P. aeruginosa PA14 CRISPR2 locus

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; crRNA under control of PermE*

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSH124-ssr

    Plasmid

    #198025

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Ap/Cb resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ApR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SmR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSH824-ssr

    Plasmid

    #198027

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Apr resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AprR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE81L-KOFP7

    Plasmid

    #160330

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2020 May 27;68(21):58

    Insert

    KOFP7
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSL3402 (pTnsABf_Pse)

    Plasmid

    #200901

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFDH

    Plasmid

    #131706

    Purpose

    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

    Depositor

    Ron Milo

    Article

    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

    Insert

    formate dehydrogenase
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSG019

    Plasmid

    #126502

    Purpose

    Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

    Depositor

    Ute Romling

    Article

    Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

    Insert

    disaggregase ClpG with 6xHis-tag
    

    Use

    Tags

    C-terminal 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTpPuc3

    Plasmid

    #62864

    Purpose

    Episome vector for Thalassiosira pseudonana (pUC-based)

    Depositor

    Hamilton Smith

    Article

    Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Episomal vector for thalassios…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

    Plasmid

    #110572

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD-stRBS-aacC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Expression

    Mutation

    Promoter

    FCP and U6

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSG020

    Plasmid

    #126503

    Purpose

    Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis

    Depositor

    Ute Romling

    Article

    Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

    Insert

    disaggregase ClpGgi with 6xHis-tag
    

    Use

    Tags

    C-terminal 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOEM-GED-EGFP

    Plasmid

    #102782

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, eGFP

    Depositor

    Elly Tanaka

    Article

    Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

    Insert

    EGFP
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCRISPR2_+1D

    Plasmid

    #149389

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOEM-GED-mCherry

    Plasmid

    #102781

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, mCherry

    Depositor

    Elly Tanaka

    Article

    Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

    Insert

    mCherry
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAGW-dpse-GAL4-DBD

    Plasmid

    #125153

    Purpose

    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    

    Use

    Gateway fly expression vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOPINF HopF2

    Plasmid

    #228397

    Purpose

    Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopF2 (hopF2 Pseudomonas syringae pv. tomato)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCRISPR2_IRdist_scram

    Plasmid

    #162319

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The distal Upstream Motif site is scrambled

    Promoter

    pBAD

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCRISPR2_-6D

    Plasmid

    #149387

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPR2_-5D

    Plasmid

    #149388

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRISPR2_+5D

    Plasmid

    #149390

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCRISPR2_+10D

    Plasmid

    #149391

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCRISPR2_IHF_Opt

    Plasmid

    #149395

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is optimized at key positions needed…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid

    #200905

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (pTarget NGS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pSL3936 (pDonor(AAVS1)_Pse)

    Plasmid

    #200903

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pHC01

    Plasmid

    #106900

    Purpose

    Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

    Depositor

    Jonathan Silberg

    Article

    Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

    Inserts

    LasR
    Methyl Halide Transferase
    Ethylene Forming Enzyme
    Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pENTR4 HopU1

    Plasmid

    #228395

    Purpose

    pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopU1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Alanine inserted after start Methionine due to Nc…

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCRISPR2_+10D+7U

    Plasmid

    #149393

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    7bp inserted upstream, and 10bp inserted downstre…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pQE30-HypocratesCS

    Plasmid

    #161957

    Purpose

    Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

    Depositor

    Dmitry Bilan

    Article

    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

    Insert

    HypocratesCS
    

    Use

    Tags

    6xHIs tag

    Expression

    Bacterial

    Mutation

    changed cysteine 355 to serine

    Promoter

    T5

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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