9,096 results

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pEGFP-C1-FLAG-Ku80

Plasmid

#46958

Purpose

Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku80 (XRCC5 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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OCT4-2A-eGFP-PGK-Puro

Plasmid

#31938

Purpose

Human OCT4 genomic targeting donor

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

OCT4 (POU5F1 Human)

Use

Targeting donor

Tags

eGFP

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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Lentiviral-FOP-dGFP-reporter

Plasmid

#14885

Depositor

Tannishtha Reya

Publication

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

mutant LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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UBQ:RUBY

Plasmid

#160909

Purpose

RUBY under the control of Ubiquitin promoter/marker for transformation

Depositor

Yunde Zhao

Publication

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and UBIQUITIN promoter

Expression

Plant

Promoter

UBIQUITIN

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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tFucci(CA)5

Plasmid

#153521

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

Depositor

Atsushi Miyawaki

Publication

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(CA)5.

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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SB100X in pCAG globin pA

Plasmid

#127909

Purpose

Sleeping Beauty Transposase expression vector driven by the CAG promoter

Depositor

Mark Groudine

Publication

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

Sleeping Beauty Transposase

Expression

Mammalian

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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CMV-Brainbow-1.0 L

Plasmid

#18721

Depositor

Joshua Sanes

Publication

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

dTomato ; mCerulean ; EYFP

Use

Cre/Lox

Expression

Mammalian

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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HA-NEDD8

Plasmid

#18711

Depositor

Edward Yeh

Publication

Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

Insert

NEDD8 (NEDD8 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pBABE-puro-mTwist

Plasmid

#1783

Depositor

Bob Weinberg

Publication

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

Twist (Twist1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCMV-PE6c

Plasmid

#207853

Purpose

Mammalian expression of PE6c prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6c

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGP-CMV-jGCaMP8s

Plasmid

#162371

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCS2+HyPer7

Plasmid

#136466

Purpose

Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Expression

Mammalian

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Publication

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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xCas9(3.7)-BE3

Plasmid

#108380

Purpose

Mammalian expression vector for xCas9(3.7)-BE3

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE3

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pML107

Plasmid

#67639

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

Depositor

John Wyrick

Publication

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3 (aka GAP promoter)

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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mTagBFP2-Lifeact-7

Plasmid

#54602

Purpose

Localization: Actin, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Lifeact-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

11 Articles

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PKC alpha WT

Plasmid

#21232

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pBigT

Plasmid

#21270

Purpose

Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

Depositor

Frank Costantini

Publication

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Type

Empty backbone

Use

Mouse Targeting

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pTREtight2

Plasmid

#19407

Depositor

Markus Ralser

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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