We narrowed to 7,666 results for: trac
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Plasmid#134169PurposeBacterial expression of RB3 stathmin-like domain (SLD)DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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pMM2-hPMCA2z/b
Plasmid#47581DepositorAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
ExpressionWormPromoterPmex-5Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2 mouse CV2 PC
Plasmid#25776DepositorAvailable SinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBC155
Plasmid#202274PurposeLevel 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent markerDepositorInsertbc182
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV105
Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway CloningExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-COX2-EBFP2
Plasmid#184720PurposeExogenous COX2DepositorInsertCOX2-EBFP2 (PTGS2 Human, Synthetic)
ExpressionMammalianAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS2365-Tier1-PhCMV-TetR
Plasmid#169566PurposeTier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).DepositorInsertPCMV-driven tetracycline repressor protein class B from transposon Tn10
ExpressionMammalianPromoterPhCMVAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV124
Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-RhoB Sensor-IRES-Blast
Plasmid#222633PurposeRho reporterDepositorInsertRhoB Sensor
UseLentiviralPromoterEF1aAvailable SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT-SEP
Plasmid#187653PurposeContains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.DepositorInsertHaloTag-SEP donor
UseAAV and CRISPRTagsN/APromoterN/AAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-FP-GFP
Plasmid#58242PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP markerDepositorInsertattR1-Cm(R)-CcdB-attR2
UseLentiviral and RNAi; Gateway destionation vectorExpressionMammalianPromoternoAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV106
Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-MyosinIIA-N-18
Plasmid#54930PurposeLocalization: Cytoskeleton, Excitation: 568, Emission: 592DepositorInsertMyosinIIA
TagsmAppleExpressionMammalianPromoterCMVAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK+-hp
Plasmid#48121PurposeShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertcodon optimized signal peptide of YngK
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV113
Plasmid#63180PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562A
Plasmid#191976PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562D
Plasmid#191977PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159847PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro
Plasmid#170466PurposeThe plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistanceDepositorArticleInsertNucleocapsid
UseLentiviralTagsmRuby3ExpressionMammalianPromoterEF1Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40
Plasmid#112496PurposeExpresses channel rhodopsin in astrocytesDepositorInsertChR2(H134R)
UseAAVTagsmCherryMutationH134RPromoterGfaABC1DAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
Plasmid#159846PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV Fos YFP MS2
Plasmid#131775PurposeFos upstream msfYFP 24xMS2 Fos downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseAAV and CRISPRPromotern/aAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcLonA
Plasmid#38330DepositorInsertLon protease fragment (lon Escherichia coli)
TagsHis, MBP, and TEVExpressionBacterialMutationPolyphosphate binding fragment of Lon protease. …PromoterT7Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
Plasmid#159866PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CRY2-mCherry
Plasmid#178525PurposeExpresses CRY2-mCherry in mammalian cellsDepositorInsertCRY2-mCherry
ExpressionMammalianPromoterCAGAvailable SinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYF-sGFAP-LOx-IRES-tdTomato
Plasmid#166919PurposeExpress the bacterial lactate oxidase (LOx) enzyme in astrocytesDepositorInsertLactate oxidase
UseLentiviralTagstdTomatoExpressionMammalianPromotersGFAPAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2
Plasmid#182355PurposemScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
Plasmid#159874PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFN_L-HiBiT
Plasmid#198319PurposeLoads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domainDepositorInsertPdp1_NTD-ZFN_L-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209660PurposeExpression of iATPSnFR2, medium affinityDepositorInsertiATPSnFR2.A95K.HaloTag
UseAAVMutationA95KPromoterGFAPAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-NEUROD1-T2A-mCherry
Plasmid#178584PurposeCre-dependent expression of NEUROD1 and mCherry under the constitutive promoterDepositorAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV114
Plasmid#63181PurposepRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
RNAseq_cal_Tuschl
Plasmid#59426PurposeEncodes sequence non-cognate to human genome for generation of RNAseq calibratorsDepositorInsertRNAseq_cal_Tuschl
Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
mRFP-dmBACCS2
Plasmid#67630PurposeDrosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switchDepositorInsertmRFP-dmBACCS2
UseDrosophila expressionPromoterDrosophila actin gene 5C gene promoterAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2
Plasmid#182347PurposeGFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-zebrafish lyn–GFP
Plasmid#35956DepositorInserthumanized zebrafish lyn (lyn Zebrafish)
TagsEGFPExpressionMammalianMutationwtPromotercmvAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159869PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB80-FRB-GFP-ppKin14VIb(861-1321)
Plasmid#174638PurposeRapalog-inducible coupling to dimeric moss kinesin-14 for retrograde transport via FRBDepositorInsertFRB-GFP-ppKin14VIb(861-1321)
TagsFRB-GFPExpressionMammalianMutationEGFP: Met1Del; ppKin14VIb(aa861-1321)PromoterChicken beta-actinAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRPromoterxyloseAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A
Plasmid#171785PurposeExpression of HaloTag N58-(GGS)X6-hH2A in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH2A
ExpressionMammalianPromoterCMVAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-CENPB-N-22
Plasmid#56325PurposeLocalization: Nucleus/Centromeres, Excitation: 515, Emission: 528DepositorInsertCENPB (CENPB Human)
TagsmVenusExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable SinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits