We narrowed to 18,496 results for: Met

1. pET26b_AT118-H-V5-His

   Plasmid

   #222850

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Tags

   V5-His

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac HT JS-Munc18c

   Plasmid

   #135555

   Purpose

   Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18c
   

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1-mTrim21

   Plasmid

   #105516

   Purpose

   Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHPHS853

   Plasmid

   #228236

   Purpose

   Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Inserts

   mCherry
   EYFP
   

   Tags

   HA and PuroR

   Expression

   Mammalian

   Promoter

   TRE3GV and none

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

   Plasmid

   #197687

   Purpose

   plasmid for expressing the DNMT3bCD

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Expression

   Bacterial

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDendra2-H2b

   Plasmid

   #157817

   Purpose

   Fluorescent reporter for histone H2B tracking

   Depositor

   Xin Chen

   Article

   Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

   Insert

   Histone H2B (H2bc3 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE3G-Bi-α-chain

   Plasmid

   #133730

   Purpose

   Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   a-Chain_gePSI
   ZsGreen1
   

   Expression

   Mammalian

   Mutation

   Only one part of the gePSI-system (aa1-141 + stop…

   Promoter

   pTRE3G-Bi

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-hfYFP

   Plasmid

   #191201

   Purpose

   Constitutive CAG-driven expression of hfYFP in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CAG

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TAC-ERN

    Plasmid

    #80475

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetOn-REEP5-mCherry-eGFP

    Plasmid

    #139870

    Purpose

    Dox-inducible lentiviral expression of human REEP5

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-G1(7FTrp)RS

    Plasmid

    #177310

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF-G1mCNPRS

    Plasmid

    #174718

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1(+)-NEMOf

    Plasmid

    #189932

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOf
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PL-SIN-EOS-C(3+)-EiP

    Plasmid

    #21313

    Purpose

    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EOS-C(3+) promoter region

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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