162,999 results

1. pBAD-HisD-miniGFP2

   Plasmid

   #188897

   Purpose

   Express 6xHis-Tag miniGFP2 in E.coli

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDEL-mScarlet3-H (aka mYongHong)

   Plasmid

   #239411

   Purpose

   Expresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   mScarlet3-H-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHnS 2comp Donor;smFP-V5 KO;Template ORF0

   Plasmid

   #240304

   Purpose

   Donor:smFP-V5 KO:Template

   Depositor

   Yuki Ogawa

   Insert

   Template gRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHnS 2comp KI;Empty

   Plasmid

   #240302

   Purpose

   Empty vector

   Depositor

   Yuki Ogawa

   Insert

   Empty gRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV 3xgRNA KO;Template

   Plasmid

   #240310

   Purpose

   KO:Template

   Depositor

   Yuki Ogawa

   Insert

   Template gRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNluc-hbb

   Plasmid

   #239753

   Purpose

   Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs.

   Depositor

   Catherine Jopling

   Article

   Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

   Insert

   Nanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 (included as part of insert)

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 8522-E06

   Plasmid

   #234291

   Purpose

   Gateway ORF Entry clone of human Tgfbr1 with stop codon (for native or N-terminal fusions); T204D mutation

   Depositor

   Dominic Esposito

   Insert

   Tgfbr1 T204D (Tgfbr1 Mouse)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   T204D

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT42

   Plasmid

   #223414

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT21

   Plasmid

   #223393

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT09

    Plasmid

    #223381

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP-GFP-Evectin-2

    Plasmid

    #221754

    Purpose

    Stable expression of Evectin-2 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PLEKHB2 (PLEKHB2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem PLEKHB2(PH domain) (amino acids 1-110)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-CMV-PE2-IRES-ZeoR

    Plasmid

    #207352

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor 2 (PE2) enzyme
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid

    #207356

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-TrpC4 [N77/15.1R]

    Plasmid

    #114504

    Purpose

    Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXBI-mClover-YTH domain

    Plasmid

    #177123

    Purpose

    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTH domain) (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    YTH domain only (IDR1 and IDR2 deletion)

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-ataxin-3 *VBM

    Plasmid

    #89976

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 282RKRR to 282HNHH

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. EasyFusion T2A-H2B-mCherry

    Plasmid

    #113089

    Purpose

    General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMP71-Flt3L

    Plasmid

    #174600

    Purpose

    Secretes the extracellular domain of murine Flt3L

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    secretes extracellular domain of mouse Flt3-L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pc DNA FRT TO- APPSwed/Ind

    Plasmid

    #114194

    Purpose

    Creation of a stable cell line expressing APP mutation (AD in vitro study)

    Depositor

    Aleksandra Radenovic

    Insert

    Amyloid-Precursor-Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Popular mouse model, overexpress 2 mutations: Swe…

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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