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Showing: 10101 - 10120 of 172695 results

  5. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

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  6. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  8. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

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  13. pAdx-EF1-tdTomato

    Plasmid
    #73354
    Purpose
    Express tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    EF1-tdTomato
    Use
    Adenoviral
    Promoter
    EF-1
    Available Since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73354-plasmid-map-sequence-id-151558

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  16. hGli1 flag3x

    Plasmid
    #84922
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84922-plasmid-map-sequence-id-422144

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  18. TFORF0432

    Plasmid
    #143877
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARHGAP35 (ARHGAP35 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143877-plasmid-map-sequence-id-471138

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Showing: 10101 - 10120 of 172695 results