166,201 results

1. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

   Plasmid

   #112927

   Purpose

   CRISPR-a reporter assay lentiviral vector (gTetO)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-BE1

   Plasmid

   #73018

   Purpose

   Expresses BE1 with N-terminal His6 tag in E. Coli

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. psd44-Lactadherin46

   Plasmid

   #46830

   Purpose

   expression of human lactadherin in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   MFGE8 (MFGE8 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK promoter

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-5X-1-LARP7

   Plasmid

   #113545

   Purpose

   Produces an N-terminal GST-tagged full length LARP7 protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   LARP7 (LARP7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTC-ApoE-Tet

   Plasmid

   #85578

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Insert

   CreER
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   ApoE.HCR.hAAT

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDAS12395_PEAR-GFP_2.0-preedited

   Plasmid

   #177185

   Purpose

   PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZJOM149

   Plasmid

   #133646

   Purpose

   Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ELO3-mCherry (ELO3 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Mutation

   Silent change from C to A at bp 834 of insert

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ad-Flag-XBP1s

   Plasmid

   #63678

   Purpose

   Adenoviral vector for XBP1s expression

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 MBP N10 TEV LIC cloning vector (2C-T)

    Plasmid

    #29706

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKII-GRAB_g5-HT3.0

    Plasmid

    #208723

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    CaMKII

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF280

    Plasmid

    #167675

    Purpose

    Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU45

    Plasmid

    #167667

    Purpose

    ENTRY vector for gRNA5 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0479 CMV-TO-VEGF-A(165) (FLP-IN)

    Plasmid

    #233384

    Purpose

    Secreted human VEGF-A

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    VEGF-A (VEGFA Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209664

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Mutation

    A95K

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRU46

    Plasmid

    #167668

    Purpose

    ENTRY vector for gRNA6 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDest-Sumo

    Plasmid

    #106980

    Purpose

    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

    Depositor

    Han Choe

    Article

    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

    Type

    Empty backbone

    Tags

    His8-Sumo

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CBh-IgKL-mNG

    Plasmid

    #183467

    Purpose

    To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    IgKL-mNeonGreen
    

    Use

    AAV

    Tags

    IgKL secretory signal

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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