We narrowed to 8,806 results for: ef1;

1. pCT-CMV-UBE3B(C1036A)-copGFP-Puro

   Plasmid

   #107344

   Purpose

   Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B(C1036A)copGFP (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   copGFP

   Expression

   Mammalian

   Mutation

   mutation C1036A

   Promoter

   CMV

   Available since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pFUSE-DEST_R1R4

   Plasmid

   #97186

   Purpose

   For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

   Depositor

   Kenneth Scott

   Article

   Lu et al Cancer Res. 2017 May 16. pii: canres.274

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SARS_COV2_orf9c_HA_BASU

   Plasmid

   #153991

   Purpose

   Express Orf9c gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf9c
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187949

   Purpose

   SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SMASh-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-BE3RA-P2A-Puro

   Plasmid

   #110839

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDF1-ADAMTS18 Wt

   Plasmid

   #29552

   Depositor

   Yardena Samuels

   Article

   Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

   Insert

   ADAMTS18 (ADAMTS18 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-BE3RA-PGK-Puro

   Plasmid

   #110843

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170996

   Purpose

   HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Myc-Idax WT

   Plasmid

   #68553

   Purpose

   This plasmid expresses full length IDAX.

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   IDAX (Cxxc4 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCT-CMV-UBE3BΔHECT-copGFP-Puro

    Plasmid

    #107343

    Purpose

    Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B(ΔHECT)-copGFP (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    deletion of HECT domain (757-end)

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLenti-BE3-P2A-GFP-PGK-Puro

    Plasmid

    #110867

    Purpose

    Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-HF1-PGK-Puro

    Plasmid

    #110857

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDF1-PTPRD-P459L

    Plasmid

    #25644

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCDF1-PTPRD-D1248N

    Plasmid

    #25646

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-BE3-PGK-Puro

    Plasmid

    #110842

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-VQR-P2A-Puro

    Plasmid

    #110848

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-BE3-P2A-Puro

    Plasmid

    #110838

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-CMV-PTPN14-CFP

    Plasmid

    #235686

    Purpose

    Express CFP tagged PTPN14 gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    PTPN14 (PTPN14 Human)
    

    Use

    Lentiviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-CMV-V5-RFP-LATS1

    Plasmid

    #235689

    Purpose

    Express RFP tagged LATS1 gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Lentiviral

    Tags

    V5-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-CMV-RPS15A-3xHA plasmid

    Plasmid

    #192245

    Purpose

    overexpression vector of RPS15A-3XHA

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    RPS15A CDS without stop coden (RPS15A Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PWPXLD-EGFR-P667A

    Plasmid

    #133750

    Purpose

    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    Mutant P667A EGFR-HA (EGFR Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    proline 667 converted to alanine (Please see depo…

    Promoter

    EF1-alpha

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-VRER-P2A-Puro

    Plasmid

    #110849

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-HF1-P2A-Puro

    Plasmid

    #110850

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-VQR-PGK-Puro

    Plasmid

    #110855

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-VRER-PGK-Puro

    Plasmid

    #110856

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDF1-ADAMTS18 (P1035S)

    Plasmid

    #29558

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 1035 to serine

    Promoter

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pCDF1-ADAM29 (S112F)

    Plasmid

    #31145

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Serine 112 changed to Pheylalnine

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pCDF1-ADAM29 (E111K)

    Plasmid

    #31144

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Glutamic acid 111 changed to Lysine

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pCDF1-ADAMTS18 (Q1002X)

    Plasmid

    #29557

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glutamine 1002 to stop codon

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pCDF1-ADAMTS18 (Q904X)

    Plasmid

    #29556

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glutamine 904 to stop codon

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pCDF1-ADAMTS18 (C638S)

    Plasmid

    #29555

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 638 to serine

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pCDF1-ADAMTS18 (G312E)

    Plasmid

    #29553

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glycine 312 to glutamic acid

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pJH104

    Plasmid

    #48262

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    TEF1p 5' fragment
    URA3 5' fragment
    TEF1p 3' fragment
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    From SK1 background. -343 to -1 relative to TEF1 …

    Promoter

    TEF1p

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EF.GFP

    Plasmid

    #17616

    Depositor

    Linzhao Cheng

    Article

    Cui et al Blood. 2002 Jan 15. 99(2):399-408.

    Insert

    EF-1a promoter (EEF1A1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO5d.EFS.SpCas9.P2A.BSD

    Plasmid

    #57821

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO5d.EFS.SpCas9.P2A.PAC

    Plasmid

    #58329

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PHBA03

    Plasmid

    #83537

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PHBA02

    Plasmid

    #83536

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 512 pME-miR

    Plasmid

    #26032

    Depositor

    Nathan Lawson

    Article

    Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

    Insert

    ef1a (eef1a1l1 Zebrafish)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

40. delCMV-mCherry

    Plasmid

    #214143

    Purpose

    Control sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. delCMV-mCitrine

    Plasmid

    #214144

    Purpose

    Volume marker / Control sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. delCMV-mCherry-2x-Rhotekin-GBD

    Plasmid

    #214142

    Purpose

    Rho activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xRhotekin-GBD (Rtkn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. delCMV-mCherry-3x-p67phox-GBD

    Plasmid

    #214141

    Purpose

    Rac activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xp67phox-GBD (NCF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pPL001

    Plasmid

    #184750

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pPL001-BBTK

    Plasmid

    #184751

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCDH-puro-cMyc

    Plasmid

    #46970

    Purpose

    lentiviral expression of c-Myc

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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