We narrowed to 18,496 results for: Met

1. pET-28a-His6-SUMO-Munc18-1wt

   Plasmid

   #135550

   Purpose

   Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18-1wt
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

   Plasmid

   #132962

   Purpose

   Firefly-luciferase single-strand annealing assay cloning backbone

   Depositor

   Gang Bao

   Article

   Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

   Insert

   Firefly Luciferase
   

   Use

   CRISPR and Luciferase

   Expression

   Mammalian

   Mutation

   Stop sequence between luciferase gene

   Promoter

   CMV

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

   Plasmid

   #219781

   Purpose

   CaST-P2A for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gal4DB-NS3-VP64-p65_IRES_mCherry

   Plasmid

   #112627

   Purpose

   Encodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

   Depositor

   John Ngo

   Article

   Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

   Insert

   Gal4DB-NS3-VP64-p65
   

   Tags

   AU1 (N term on NS3), HA (C term on NS3), and T7 (…

   Expression

   Mammalian

   Mutation

   NS3 mutant T54A

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-ChRger3-TS-EYFP

   Plasmid

   #127240

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR-CyOFP1

   Plasmid

   #228604

   Purpose

   Mammalian expression of CyOFP1, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   CyOFP1
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC-SARS-CoV-2 NSP3

   Plasmid

   #158354

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP3 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

   Plasmid

   #193823

   Purpose

   CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   [4xCox8]-ChemoG-NAD
   

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NDV-HN-SpyTag

   Plasmid

   #160000

   Purpose

   Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   NDV-HN-SpyTag
   

   Tags

   His6-tag

   Expression

   Mammalian

   Promoter

   Chicken beta-actin promoter

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo:alpha4-mcherry

    Plasmid

    #45073

    Depositor

    Henry Lester

    Article

    Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

    Insert

    alpha4-mcherry (Chrna4 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-His6-ODC-Ctag

    Plasmid

    #163614

    Purpose

    Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    ODC (ODC1 Human)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHOHO2_Hsp82viiA

    Plasmid

    #61708

    Purpose

    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

    Depositor

    Dan Bolon

    Article

    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

    Insert

    Hsp82viiA (HSP82 Budding Yeast)
    

    Use

    Yeast integration vector targeting inserts to the…

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Heteromeric coiled coil inserted after amino acid…

    Promoter

    TEF1

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCX-NTOM-SpiCee

    Plasmid

    #140902

    Purpose

    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    NTOM-SpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

    Plasmid

    #169533

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVSL10

    Plasmid

    #201182

    Purpose

    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

    Depositor

    Jack Szostak

    Article

    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

    Insert

    Bst polymerase large fragment (BF) F710Y/D598A
    

    Tags

    HRV 3C protease cleavage site and His6

    Expression

    Bacterial

    Mutation

    F710Y D598A

    Promoter

    T5-lacO

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-HA-UFM1-dCS

    Plasmid

    #139869

    Purpose

    Mammalian expression of HA-tagged human UFM1-cCS

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFM1 (UFM1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    d84-85

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-hygro LAP2

    Plasmid

    #15740

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LAP2 (CEBPB Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIFR-3

    Plasmid

    #233295

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Promoter

    p14G

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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