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Plasmid#127505PurposePlasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKAM-GFP
Plasmid#101865PurposeLentiviral empty backbone, EGFP markerDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEf1aAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luciferase
Plasmid#21375Purposeself-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and LuciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
ER-M-TAG (pGS555)
Plasmid#160040PurposepLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertMMGT1-mScarlet-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAM-BFP-Puro
Plasmid#101864PurposeLentiviral empty backbone, dual BFP and puro markersDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307
Plasmid#41392PurposeConstitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-m5HT6-EGFP
Plasmid#200443PurposeLentiviral expression of mouse m5HT6-EGFPDepositorInsert5HT6-EGFP
UseLentiviralTagsEGFPExpressionMutationPromoterEF1αAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymNeongreen
Plasmid#193959PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeastDepositorInsertymNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymScarletI
Plasmid#193960PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeastDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymTurquoise2
Plasmid#193958PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeastDepositorInsertymTurquoise2
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
R777-E225 Hs.RAPGEF2
Plasmid#70509PurposeGateway ORF clone of human RAPGEF2 [NM_014247.2] with stop codon (for native or N-terminal fusions)DepositorInsertRAPGEF2 (RAPGEF2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJR288
Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
R777-E226 Hs.RAPGEF2-nostop
Plasmid#70510PurposeGateway ORF clone of human RAPGEF2 [NM_014247.2] without stop codon (for C-terminal fusions)DepositorInsertRAPGEF2 (RAPGEF2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCas9-miniTurbo
Plasmid#219822PurposeEF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)DepositorInsertdCas9
UseCRISPRTagsminiTurboExpressionMammalianMutationD10A/H841APromoterEF1aAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAM10
Plasmid#198743PurposepEF1-GFP, Geneticin resistanceDepositorInsertGFP
UseAcanthamoeba expressionTagsExpressionMutationPromoterpEF1Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK013
Plasmid#65120PurposeEncodes pTEF1 as a Type 2 part to be used in the Dueber YTK systemDepositorInsertpTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-dCasRx
Plasmid#219821PurposeEF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)DepositorInsertdCasRx
UseCRISPRTagsminiTurboExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Aiolos in Artichoke
Plasmid#74452PurposeMonitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorInsertAiolos (IKZF3) (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterSFFV and EF1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSPR33
Plasmid#217318PurposeTEF1(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH0007
Plasmid#174162PurposeUCOE-EF1A-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY.005
Plasmid#228684PurposepTEF1 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF1
UseTagsExpressionYeastMutationWTPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR27
Plasmid#217312PurposeTEF1(p)-amdS:mRuby3 fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR29
Plasmid#217314PurposeTEF1(p)-amdS:mNeonGreen fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Km
Plasmid#46813PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Ph
Plasmid#46814PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km
Plasmid#46815PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Km
Plasmid#46817PurposeTEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSPR31
Plasmid#217316PurposeTEF1(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Ph
Plasmid#46816PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-3xFLAG/HA/NBL10
Plasmid#110568PurposeCre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tagDepositorInsert3xFLAG/HA-tagged anti-GFP VHH
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Ph
Plasmid#46818PurposeTEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSSa23
Plasmid#177373PurposeExpresses red-shifted luciferase under TEF1 promoter in Candida glabrataDepositorInsertCgLUCopt-mut2
UseTagsExpressionMutationPromoterTEF1Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM mir shHoxB9 b
Plasmid#27020DepositorInsertmir HoxB9 3b
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMYT026
Plasmid#180679PurposeEncodes tTEF1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInserttTEF1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIR3DH8
Plasmid#165068PurposeKnocking out GAL80 using KlURA3 markerDepositorInsertgal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR-wt
Plasmid#153339PurposeWild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoterDepositorInsertVanR
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM mir shHoxB9 a
Plasmid#27021DepositorInsertmir HoxB9 2A
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJA291
Plasmid#74487PurposeeF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFPDepositorInsertpuroR-mCherry-T2A-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1511
Plasmid#62365PurposepEF1a-puro-T2A-ATHmmuRPL10ADepositorInsertPuro-T2A-AVITEVHAmmuRPL10A
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Aiolos AA130-189 in Artichoke
Plasmid#74451PurposeMonitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorInsertAiolos (IKZF3) AA130-189 (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFPY2A
Plasmid#165066PurposeCharacterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFPDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV21
Plasmid#128880PurposePosition 2 module- Tef1 promoterDepositorInsertTef1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF738
Plasmid#122032PurposeEF1a-WNV-protease-GFPDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD426
Plasmid#202079PurposeFrame +1/-0 selector for NHEJ knock-inDepositorInsertpEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD425
Plasmid#202078PurposeFrame +0 selector for NHEJ knock-inDepositorInsertpEF1a-mCherry-Cas9 + Frame +0 sgRNA
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD427
Plasmid#202080PurposeFrame +2/-1 selector for NHEJ knock-inDepositorInsertpEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYS19
Plasmid#202084PurposeFrame -2 selector for NHEJ knock-inDepositorInsertpEF1a-mCherry-Cas9 + Frame –2 sgRNA
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only