We narrowed to 39,129 results for: Eras
-
Plasmid#201585PurposeExpresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-542 of DNA polymerase kap…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1.M10.luc
Plasmid#193706PurposeExpressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)DepositorInsertH1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TERTp124_Gluc_CCR5
Plasmid#192271PurposeDonor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutation-124 C to TPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TERTp146_Gluc_CCR5
Plasmid#192272PurposeDonor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutation-146 C to TPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH862-CEN-slowmaxRLuc/minCFLuc
Plasmid#115371PurposeExpresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferaseDepositorInsertsFirefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)
UseTagsExpressionYeastMutationAll codons have been changed for the most favoura…PromoterADH1 and TDH3Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorInsertDNMT1 (DNMT1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXJ-CD274-Nluc
Plasmid#232658PurposeNluc-tagged PD-L1 expresion in mammalian cells; lectin-captured luciferase reporter assayDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSUMO ACD11
Plasmid#170738PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertacd11 (ACD11 Mustard Weed)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF3
Plasmid#69046Purposeexpression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF3 (IKZF3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterIRESAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Nluc-_(PLRSKK)-CA1-19
Plasmid#232657PurposeNluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assayDepositorInsertCGA (CGA Human)
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRM919_WT aa 1-419 His POLI
Plasmid#201445PurposeLow copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLIDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 1-449 of DNA polymerase iot…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLuc-OptoJNKi
Plasmid#89744PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_EIF2B2:1-351
Plasmid#211083PurposeMammalian expression of full-length human EIF2B2. C-terminal NanoLuc luciferase tag.DepositorInsertEIF2B2:M1-L351 (EIF2B2 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailable sinceDec. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR4_miniFXN_8
Plasmid#234361PurposeMini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.DepositorInsertFXN (FXN Human)
UseCloningTagsFlag TagExpressionMutationThere is a partial deletion in the 5'UTR of …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-420 POLI
Plasmid#131234PurposeMammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S433A POLI
Plasmid#131235PurposeMammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only