169,365 results

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-HP1alpha

Plasmid

#179701

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Flag-humanRBM47

Plasmid

#134607

Purpose

Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

Flag-humanRBM47 (RBM47 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pP2-phiV10

Plasmid

#196333

Purpose

Plasmid expressing chimeric P2-phiV10 tail fiber

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-phiV10

Expression

Bacterial

Promoter

P2 late promoter

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-CybGam-WPRE

Plasmid

#207662

Purpose

Control for CybSEP2

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

Gamillus

Use

AAV

Mutation

Histidine 86 and 159 to alanines, inserted gamill…

Promoter

hSynapsin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-Integrin-Beta1-N-18

Plasmid

#58105

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG SARS-CoV2 RdRp.co

Plasmid

#154008

Purpose

Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

Depositor

Patrick Moore

Article

Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

Insert

Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)

Tags

3xFLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac HTB MBP-SARM1

Plasmid

#195763

Purpose

Purification of SARM1 from insect cells

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Tags

HIS-MBP-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DIO-NaChBac-dTomato

Plasmid

#236252

Purpose

Cre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

hSyn

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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Go1-CASE

Plasmid

#168123

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at L91/G92 within GNAO1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1688

Plasmid

#144371

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HNF1A (HNF1A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-puro

Plasmid

#167828

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#236407

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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TfR-pHuji

Plasmid

#61505

Purpose

Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Tags

pHuji

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-FGF21

Plasmid

#182407

Purpose

Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-fgf21 (FGF21 Human)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP1D1-Strep-II

Plasmid

#224209

Purpose

Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

Depositor

Anne Robinson

Article

Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

Insert

MSP1D1-Strep-II

Tags

Strep-II Tag

Expression

Bacterial

Mutation

Substitution of 7xHis tag found in pMSP1D1 for a …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT1_WT

Plasmid

#82234

Purpose

Gateway Donor vector containing STAT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT1 (STAT1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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