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pnYC-NpM-HsPAN3_245-687_T

Plasmid

#147572

Purpose

Bacterial Expression of HsPAN3_245-687

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

HsPAN3_245-687 (PAN3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0022

Plasmid

#197517

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtDREB26 (AT1G21910) 1000 bp upstream of TSS

Use

Synthetic Biology

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0024

Plasmid

#197518

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNLP7 (AT4G24020) 1000 bp upstream of TSS

Use

Synthetic Biology

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0025

Plasmid

#197519

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNIR1 (AT2G15620) 936 bp upstream of TSS

Use

Synthetic Biology

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0020

Plasmid

#196163

Purpose

Phytobricks Level 0 part: AtNLP6 promoter 1kb upstream

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNLP6 promoter 1kb upstream

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0014

Plasmid

#196162

Purpose

Phytobricks Level 0 part: ANAC032 promoter 1kb upstream

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

ANAC032 promoter 1kb upstream

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0018

Plasmid

#196164

Purpose

Phytobricks Level 0 part: AtARF18 promoter 1kb upstream

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtARF18 promoter 1kb upstream

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A-CC-Di

Plasmid

#190707

Purpose

Encodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A-CC-Di

Mutation

V150A

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC-N-Venus-V150A

Plasmid

#190708

Purpose

Control plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

N-Venus-V150A

Mutation

V150A

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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GB3270

Plasmid

#193114

Purpose

Random sequence R3 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R3

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GB3269

Plasmid

#193113

Purpose

Random sequence R2 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R2

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GB2815

Plasmid

#193112

Purpose

Random sequence R1 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R1

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2At(2nd)

Plasmid

#183085

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2At(2nd)

Plasmid

#183086

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2At(2nd)

Plasmid

#183087

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA-HA

Plasmid

#186374

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-HA-RfA

Plasmid

#186373

Purpose

Destination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-Venus-RfA

Plasmid

#186371

Purpose

Destination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Chimera

Plasmid

#188802

Purpose

Chimeric Avs4 with transmembrane effector

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4 chimera

Expression

Bacterial

Promoter

EcAvs4 native promoter

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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