We narrowed to 7,692 results for: Lif;
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Plasmid#123353PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertCerulean3-Cerulean3-FLARE-Cameleon
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianPromoterCMVAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC150-RFP-EGFP-LGALS3 R186S
Plasmid#175779PurposePiggyBac Vector expressing GFP-RFP-LGALS3 R186SDepositorAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 delta PB1 silent
Plasmid#187985Purposehuman p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552DepositorInsertp62 (NUP62 Human)
TagsmCherryExpressionMammalianMutationLacking PB1 domain (aa1-102) with a silent mutati…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-AKAR
Plasmid#123333PurposeRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertmCherry-mCherry-FLARE-AKAR
Tags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHalo-MyosinIIA-C25
Plasmid#128573PurposeStudies of cellular contractilityDepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p8328 pLIX YAP1 S94A
Plasmid#184529PurposeExpression of YAP1 S94ADepositorAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-Venus-FLARE-Cameleon
Plasmid#123351PurposeYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertVenus-Venus-FLARE-Cameleon
Tags6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11-RinA)2
Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsR, PhrpLE, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580AAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm
Plasmid#185676PurposeSpCas9 and gRNA targeting the C-terminus of HsIRE1DepositorInsertERN1 gRNA (ERN1 Human)
UseCRISPRAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema6a.a-AP-His
Plasmid#72037PurposeExpresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6b(L)-AP-His
Plasmid#72039PurposeExpresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6b(S)-AP-His
Plasmid#72040PurposeExpresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3xVHH05-DsRed
Plasmid#171580PurposeFor scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mMyh9 - 2
Plasmid#198492Purposelentiviral stable expression of mMyh9 gRNA 2DepositorAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mMyh9 - 1
Plasmid#198491Purposelentiviral stable expression of mMyh9 gRNA 1DepositorAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP2s
Plasmid#101276PurposeGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1DepositorInsertASAP2s
UseAAVExpressionMammalianPromoterhSyn (human synapsin)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema7a-Fc-His
Plasmid#72175PurposeExpresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40
Plasmid#100855PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoterDepositorInsertjRGECO1b
UseAAVExpressionMammalianPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T373
Plasmid#58912Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restoredDepositorInsertRb (RB1 Human)
TagsHAExpressionMammalianMutationdelta CDK* with T373 restoredPromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3x127D01-DsRed
Plasmid#171578PurposeFor scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTMiniTrex-mCherry-Shark2
Plasmid#233144PurposeServes as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassetteDepositorInsertsmCherry
Shark2
UseProkaryotic expressionTags3x Ty tagMutationCodon optimized for Trypanosoma cruziAvailable SinceMarch 20, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
SspB-HaloTag-DHPH-TIAM1
Plasmid#176114PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3xVHH05-DsRed
Plasmid#171581PurposeFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-SnFR-gamma2-minWPRE
Plasmid#165497PurposeAAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisationDepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
UseAAVPromoterhuman SynapsinAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SspB-mCherry-DHPH-TIAM1
Plasmid#176104PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plxna2-AP-His
Plasmid#71997PurposeExpresses the extracellular region of the PlexinA2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S612
Plasmid#58914Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restoredDepositorInsertRb (RB1 Human)
TagsHAExpressionMammalianMutationdelta CDK* with S612 restoredPromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S608
Plasmid#58913Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restoredDepositorInsertRb (RB1 Human)
TagsHAExpressionMammalianMutationdelta CDK* with S608 restoredPromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ncam1_1.18.1-Fc-His
Plasmid#72088PurposeExpresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40
Plasmid#100856PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorInsertjRGECO1b
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.3-AP-His
Plasmid#71974PurposeExpresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shQKI-3371
Plasmid#115455PurposeConstitutive lentiviral expressionDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Unc5b.a-Fc-His
Plasmid#72178PurposeExpresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:Lamp1-mGFP
Plasmid#192356PurposeLamp1 tagged with mGFP, expressed under the UAS promoterDepositorAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-AP-His
Plasmid#71995PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3a(L, del3)-Fc-His
Plasmid#72136PurposeExpresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Phi3-Zsgreen-PGK-mCherry
Plasmid#153320PurposeExpresses zsGreen and mCherry in mammalian cells.DepositorInsertzsGreen
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemExpressionMammalianMutationY64L (mutRFP)Available SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema3a(S)-Fc-His
Plasmid#72138PurposeExpresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.a-AP-His
Plasmid#71984PurposeExpresses the extracellular region of the Netrin G1, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
FastLightR-bRaf-mVenus
Plasmid#162155PurposeExpresses FastLightR-bRaf-mVenus in mammalian cellsDepositorInsertFastLightR-bRaf-mVenus
TagsmVenusExpressionMammalianMutationValine 600 is substituted with Glutamic acidPromoterCMVAvailable SinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin-GFP
Plasmid#186138PurposeFor expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteriaDepositorAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E)
Plasmid#187870PurposeGal4/UAS expression of dSarm(isoE)DepositorInsertdSarm isoform E (Sarm Fly)
Available SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Neo1.a-Fc-His
Plasmid#72089PurposeExpresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSII-PPM1H D288A-mApple
Plasmid#198474PurposeMammalian expression of PPM1H-mAppleDepositorAvailable SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only