We narrowed to 8,806 results for: ef1;
-
Plasmid#31799DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glycine 561 to glutamic acidPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRERRA-PGK-Puro
Plasmid#110859PurposeLentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)DepositorInsertCas9-VRERRA
UseLentiviralTagsFLAGExpressionMutationD1135V, G1218R, R1335E, T1337R, and NLS sequence …PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRERRA-P2A-GFP-PGK-Puro
Plasmid#110865PurposeLentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-VRERRA
UseLentiviralTagsFLAGExpressionMutationD1135V, G1218R, R1335E, T1337R, and NLS sequence …PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRERRA-P2A-Puro
Plasmid#110852PurposeLentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)DepositorInsertCas9-VRERRA
UseLentiviralTagsFLAGExpressionMutationD1135V, G1218R, R1335E, T1337R, and NLS sequence …PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-RFP-YAP (isoform 5)
Plasmid#235690PurposeExpress RFP tagged YAP gene in mammalian cells using the CMV promoterDepositorInsertYAP (YAP1 Human)
UseLentiviralTagsRFPExpressionMammalianMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-P2A-GFP-PGK-Puro
Plasmid#110862PurposeLentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-P2A-GFP-PGK-Puro
Plasmid#110863PurposeLentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGExpressionMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13A7
Plasmid#223837PurposeExpressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiaeDepositorInsertpTEF1>mScarletI3>
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13B7
Plasmid#223838PurposeExpressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiaeDepositorInsertpTEF1>StayGold>
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILFPmTagBFP2FrFAST
Plasmid#223839PurposeExpressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiaeDepositorInsertpTEF1>mTagBFP2>FrFAST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-C-RG
Plasmid#49101PurposeCre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under EF1a promoter, in AAV virusDepositorInsertCerulean-E2A-RG
UseAAV and Cre/LoxTagsceruleanExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1F5
Plasmid#165064PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminatorDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
iC30 PB-Zim3-dCas9-P2A-BFP_hygro
Plasmid#236743PurposeConstitutive expression of Zim3-dCas9InsertsCAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
EF1a-hygro-WPRE
UseTagsExpressionMammalianMutationPromoterCAG and EF1aAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1H5
Plasmid#165065PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF710
Plasmid#121892PurposeEF1a-WNV-protease-mTagBFP2DepositorInsertsUseLentiviralTagsExpressionMutationPromoterEF1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF709
Plasmid#121891PurposeEF1a-ZIKV-protease-mTagBFP2DepositorInsertsUseLentiviralTagsExpressionMutationPromoterEF1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGY240
Plasmid#239811PurposeExpress H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungiDepositorInsertsH3
H2B
UseFungal expressionTagsEGFP and mRFP1ExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY245
Plasmid#239812PurposeExpress H3-NLS-EGFP in filamentous fungiDepositorInsertNLS of H3
UseFungal expressionTagsEGFPExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY251
Plasmid#239813PurposeExpress H2B-NLS-EGFP in filamentous fungiDepositorInsertNLS of H2B
UseFungal expressionTagsEGFPExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY199
Plasmid#239801PurposeExpress EGFP in filamentous fungiDepositorInsertEGFP
UseFungal expressionTagsExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY203
Plasmid#239802PurposeExpress GUS in filamentous fungiDepositorInsertGUS
UseFungal expressionTagsExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY208
Plasmid#239803PurposeExpress mCherry in filamentous fungiDepositorInsertmCherry
UseFungal expressionTagsExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY209
Plasmid#239804PurposeExpress mRFP1 in filamentous fungiDepositorInsertmRFP1
UseFungal expressionTagsExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY214
Plasmid#239805PurposeExpress EGFP and mRFP1 simultaneously in filamentous fungiDepositorInsertsEGFP
mRFP1
UseFungal expressionTagsExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY219
Plasmid#239806PurposeExpress H2B-EGFP in filamentous fungiDepositorInsertH2B
UseFungal expressionTagsEGFPExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY220
Plasmid#239807PurposeExpress H3-EGFP in filamentous fungiDepositorInsertH3
UseFungal expressionTagsEGFPExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY224
Plasmid#239808PurposeExpress H2B-mRFP1 in filamentous fungiDepositorInsertH2B
UseFungal expressionTagsmRFP1ExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY225
Plasmid#239809PurposeExpress H3-mRFP1 in filamentous fungiDepositorInsertH3
UseFungal expressionTagsmRFP1ExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY232
Plasmid#239810PurposeExpress EGFP-P2A-mRFP1 in filamentous fungiDepositorInsertsEGFP
mRFP1
UseFungal expressionTags2AExpressionMutationPromoterTef1INAvailable sinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
L0-AA008
Plasmid#185754PurposeLevel 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).DepositorInsertpAaEF1a (promoter)
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMST-1313
Plasmid#118076PurposepTEF1-cexA-tCYC1DepositorInsertpTEF1-cexA-tCYC1
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHBA01
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Fon/ConN2cG
Plasmid#234709PurposepAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta GDepositorInsertFon Con N2cG
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AYBEv0.1
Plasmid#193966PurposeA wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(wt)
UseTagsExpressionMammalianMutationPromoterhU6, EF1a, CBhAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AYBEv3
Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
UseTagsExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MZB222
Plasmid#221772PurposeBacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPasesDepositorInsertArfGEF1 (ARFGEF1 Human)
UseTags10xHis-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUDC175
Plasmid#103019PurposeS. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoterDepositorInsertFncpf1
UseTags3HA and NLSExpressionYeastMutationhuman codon optimizedPromoterTEF1Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only