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Neo-A OR T OR C-SpyTag

Plasmid

#246948

Purpose

Expresses multifunctional construct for OR OR-gated release of Neoleukin (Neo) from materials in response to sortase eSrtA(2A9) OR TEV OR HRV-3C treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199

Insert

Please see depositor comments below

Tags

6x His tag

Expression

Bacterial

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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mGL-A AND T AND C-SpyTag

Plasmid

#246949

Purpose

Expresses multifunctional construct for AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV AND HRV-3C treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199

Insert

Please see depositor comments below

Tags

6x His tag

Expression

Bacterial

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SlmiR482bTS-B/c

Plasmid

#234368

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - Subunit C

Plasmid

#233131

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1397-DddtoxA-C

Plasmid

#191583

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1397-DddtoxA-C

Plasmid

#191584

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1333-DddtoxA-C

Plasmid

#191587

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NI_G1333-DddtoxA-C

Plasmid

#191588

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NN_G1333-DddtoxA-C

Plasmid

#191589

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1397-DddtoxA-C

Plasmid

#191593

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NI_G1397-DddtoxA-C

Plasmid

#191594

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NN_G1397-DddtoxA-C

Plasmid

#191595

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1333-DddtoxA-C

Plasmid

#191579

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1333-DddtoxA-C

Plasmid

#191580

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMRBad-C-DiB-RM

Plasmid

#168478

Purpose

C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

C-fragment of DiB-RM

Expression

Bacterial

Promoter

araBAD

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMRBad-Z-C-wtBlc

Plasmid

#168256

Purpose

C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly

Depositor

Jens Meiler

Article

Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

Insert

Leucine Zipper + C-fragment of wtBlc

Expression

Bacterial

Promoter

araBAD

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmEGFP-C

Plasmid

#113750

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmRuby-C

Plasmid

#113756

Purpose

Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

2xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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∆C' terminal-DPYSL2-pLJC2

Plasmid

#189867

Purpose

Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

ΔC-DPYSL2

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

deleted the C'terminus of DPYSL2 (491-572 aa)

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

Plasmid

#171726

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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