We narrowed to 15,506 results for: LIS
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Plasmid#53276PurposeContains ctrE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and the lcyE gene of Lactuca sativa and thereby produces epsilon-carotene in Escherichia coliDepositorInsertlcyE
UseLow copy number bacterial cloning vectorPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
TagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHTganA-Cpf1
Plasmid#158647PurposeConstitutive transcription of FnCpf1 and ganA crRNADepositorInsertCpf1, ganA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…ExpressionBacterialPromoterP43Available SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
ExpressionMammalianMutationnoneAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBFD1Bad
Plasmid#53564PurposeProduces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinoseDepositorInserthbfd1 cDNA
ExpressionBacterialPromoteraraBADAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMasterE
Plasmid#165080PurposeEpisomal expression of reprogramming factors.DepositorInsertsUseEpisomalExpressionMammalianAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-RNase HIII
Plasmid#12683DepositorInsertRNase HIII
TagsHisExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLY098-RetroEFS-BCMABBz-WPRE
Plasmid#192199PurposeRetro-BCMACARDepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
RHPMP126 (mOrange2-alpha Tubulin)
Plasmid#88835PurposeLocalization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2DepositorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-94N
Plasmid#53281PurposeContains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthaseDepositorInsertcrtY, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-pHAT
Plasmid#83928PurposehsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable SinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_membrane_EGFP
Plasmid#139402Purposein vitro transcription for mRNADepositorInsertmembrane-EGFP
UseIn vitro transcriptionTagsEGFPPromoterT3Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSV40_HA-mPKR
Plasmid#120538Purposeprotein expressionDepositorInsertPKR (Eif2ak2 Mouse)
ExpressionMammalianAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE
Plasmid#192204PurposeLenti-GL(GFP-Luciferase)DepositorInsertLenti-GL(GFP-Luciferase)
UseLentiviral; Mammalian expressionMutationNAAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)
Plasmid#192190PurposeHER2 CAR AAV vector PRODH2 KI (pLY080b)DepositorInsertHER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
UseAAV; Mammalian expressionMutationNAAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro2 E208K/E328K
Plasmid#47900PurposeExpresses myc tagged Miro2 V208K/E328K mutantDepositorInsertMiro2 E208K/E328K (RHOT2 Human)
TagsmycExpressionMammalianMutationE208K/E328K, abolishes calcium bindingPromoterCMVAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV7 mitoDendra2
Plasmid#157757PurposeExpression of the fluorescent protein Dendra2 targeted to mitochondriaDepositorInsertDendra2
ExpressionMammalianMutationMTS coxVIII +Dendra2PromoterCMVAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-EGFP
Plasmid#108896PurposeMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertAPEX2-P2A-EGFP
UseGateway CloningAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85844PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorInsertArcLightCo-Q239
TagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterhSyn1Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only