We narrowed to 92,138 results for: lias

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MTRAP-bio

Plasmid

#68530

Purpose

Expresses full-length P. vivax MTRAP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

MTRAP

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_1

Plasmid

#74180

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pX330 N-FKBP-Cas9

Plasmid

#162727

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS

Plasmid

#154768

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9-RBMX

Plasmid

#163565

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PVX_110945-bio

Plasmid

#68538

Purpose

Expresses full-length P. vivax PVX_110945 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

PVX_110945

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

Plasmid

#102892

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-Stim1-ΔCTD

Plasmid

#186621

Purpose

Encodes Stim1 with C-terminal domain deletion fused to YFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Deletion of C-terminal domain 601 to 685

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN delta N-term

Plasmid

#40932

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-220

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
MSP1-bio

Plasmid

#68505

Purpose

Expresses full-length P. vivax MSP1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

MSP1

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2-T2A-HO1

Plasmid

#179485

Purpose

Expresses miRFP2 and HO1in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmIRFP2-T2A-HO1

Expression

Mammalian

Mutation

NO

Promoter

tag-168 promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM5-mDPYD

Plasmid

#61614

Purpose

The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidine dehydrogenase (Dpyd Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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ABE8.8-d

Plasmid

#136295

Purpose

expresses ABE8.8-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha delta C-term

Plasmid

#40930

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 296-600

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-3XFLAG-Ubac2

Plasmid

#123597

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

ubiquitin associated domain containing 2 (Ubac2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pODN-AAVS1-mR2-CAAX

Plasmid

#183871

Purpose

Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)

Use

CRISPR; Donor template

Tags

mRuby2-CAAX

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

CMV

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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RIPR-bio

Plasmid

#68526

Purpose

Expresses full-length P. vivax RIPR ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

RIPR

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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DBP-bio

Plasmid

#68528

Purpose

Expresses full-length P. vivax DBP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

DBP

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-WDR34

Plasmid

#66797

Purpose

Expression of human WDR34 tagged with GFP under the control of CMV

Depositor

Lotte Pedersen

Article

Goggolidou et al Development. 2014 Oct;141(20):3966-77. d

Insert

WDR34 (WDR34 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only