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We narrowed to 3,985 results for: LYC

Showing: 2701 - 2750 of 3985 results
  1. A3A-PBE-ΔUGI

    Plasmid
    #119770
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)
    Depositor
    Insert
    APOBEC3A-nCas9-ΔUGI-NLS
    Use
    Tags
    NLS
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pExT

    Plasmid
    #36889
    Depositor
    Inserts
    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides before nucleotide 275 and ins…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
  3. pExG

    Plasmid
    #36888
    Depositor
    Inserts
    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
  4. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Insert
    Smad family member 4 (SMAD4 Human, Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pCS5

    Plasmid
    #55750
    Purpose
    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 59-264 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid
    #167169
    Purpose
    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Leucine 173 to Alanine; changed Glycine 2…
    Promoter
    CMV promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pDGE663

    Plasmid
    #153261
    Purpose
    M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pDGE665

    Plasmid
    #153263
    Purpose
    M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pDGE654

    Plasmid
    #153252
    Purpose
    M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

    Plasmid
    #171726
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. E3_pENTR_L3-L2_NG_G1397-DddtoxA-C

    Plasmid
    #171734
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

    Plasmid
    #171735
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

    Plasmid
    #171727
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

    Plasmid
    #171731
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. E1_pENTR_L1-L4_NN_G1397-DddtoxA-C

    Plasmid
    #171730
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. RAMA-bio-His

    Plasmid
    #50737
    Purpose
    Expresses enzymatically monobiotinylated full length RAMA ectodomain
    Depositor
    Insert
    RAMA
    Use
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
  17. miR-144 Trap

    Plasmid
    #60934
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Insert
    miR-144 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mutation
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  18. pMS1088

    Plasmid
    #80159
    Purpose
    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Insert
    HisSUMO-Cys-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  19. DPAGT1 (DPAGT1A-c005)

    Plasmid
    #98228
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Insert
    DPAGT1A (DPAGT1 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    pfam00953:Glycos_transf_4 98-275
    Promoter
    polyhedrin
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  20. pDGE660

    Plasmid
    #153258
    Purpose
    M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  21. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid
    #162949
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

    Plasmid
    #162918
    Purpose
    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)
    Depositor
    Insert
    firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. pVH028

    Plasmid
    #80393
    Purpose
    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
    Depositor
    Inserts
    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to 4 SH3 domains
    Expression
    Bacterial
    Mutation
    Glycine 448 changed to Alanine, makes it primaril…
    Promoter
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. pMS1251

    Plasmid
    #80163
    Purpose
    pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Insert
    HisSUMO-Ald-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  25. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Use
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 2701 - 2750 of 3985 results