We narrowed to 3,985 results for: LYC
-
Plasmid#158371PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 S (S )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1 shTGFBR3 puro
Plasmid#58696PurposeLentiviral shRNA vector for knockdown of human TGFBR3DepositorInsertshTGFBR3 (TGFBR3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pLKO.1-puro-PARG shRNA
Plasmid#23260DepositorInsertpoly(ADP-ribose) glycohydrolase shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pgWIZ-SGCAgl
Plasmid#131456PurposeEncodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imagingDepositorInsertSGCA (SGCA Human)
UseTags3xFLAGExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExT
Plasmid#36889DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pExG
Plasmid#36888DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Hygro
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Smad4-1719
Plasmid#49383PurposeInsert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertSmad family member 4 (SMAD4 Human, Mouse)
UseLuciferaseTagsExpressionMutationPromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (G244R)
Plasmid#31155DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationGlycine 244 changed to ArgininePromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmRPS27a
Plasmid#69559PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Human, Mouse)
UseTagsExpressionBacterialMutationChanged Alanine 133 (human RPS27a) to Glycine (mo…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP ratAPOBEC1 L173A/G227A
Plasmid#167169PurposeExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.DepositorArticleInsertAPOBEC1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
UseTagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE663
Plasmid#153261PurposeM6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE665
Plasmid#153263PurposeM8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE654
Plasmid#153252PurposeM1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB1omega2_SF (GB0466)
Plasmid#68243PurposeTwister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SF (GB0107)
Plasmid#68233PurposeTwister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddtoxA-C
Plasmid#171726PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddtoxA-C
Plasmid#171734PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddtoxA-N
Plasmid#171735PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddtoxA-N
Plasmid#171727PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddtoxA-N
Plasmid#171731PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddtoxA-C
Plasmid#171730PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAMA-bio-His
Plasmid#50737PurposeExpresses enzymatically monobiotinylated full length RAMA ectodomainDepositorInsertRAMA
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
miR-144 Trap
Plasmid#60934Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-144 Trap
UseLentiviralTagsGFPExpressionMutationPromoterbidirectional phosphoglycerate kinase promoter (b…Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pMS1088
Plasmid#80159PurposepET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Cys-4xNuG2-Ald
UseTagsHisSUMOExpressionMutationPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
DPAGT1 (DPAGT1A-c005)
Plasmid#98228PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPAGT1A (DPAGT1 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00953:Glycos_transf_4 98-275PromoterpolyhedrinAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE660
Plasmid#153258PurposeM4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
C513-E07: attB5r-<p10p-polyhedrinp>-attB1r
Plasmid#162949PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3
Plasmid#162918PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase/green fluorescent protein fusion with upstream IRES sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
CBM9-ID-H1
Plasmid#171709PurposeExpresses SARS-CoV-2 Spike epitopeDepositorInsertSARS-CoV-2 Spike protein (S SARS-CoV-2)
UseTagsCBM-9ExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVH028
Plasmid#80393PurposeContains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)DepositorUseSynthetic BiologyTagsFused by GS linkers to 4 SH3 domainsExpressionBacterialMutationGlycine 448 changed to Alanine, makes it primaril…PromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO pGK
Plasmid#68452PurposeTOPO construct expressing pGK for generation of GMAP-compatible promoterDepositorInsertPhosphoglycerate Kinase 1 Promoter (PGK1 Human)
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFA0445w-bio-His
Plasmid#50811PurposeExpresses enzymatically monobiotinylated full length PFA0445wDepositorInsertPFA0445w
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PF10_0166-bio-His
Plasmid#50801PurposeExpresses enzymatically monobiotinylated full length PF10_0166DepositorInsertPF10_0166
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His mICA NG
Plasmid#70162PurposeExpresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydraseDepositorInsertmouse inhibitor of carbonic anhydrase
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn470Asp and Asn645AspPromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS1251
Plasmid#80163PurposepET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Ald-4xNuG2-Ald
UseTagsHisSUMOExpressionMutationPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC-hTDG-K330A-IntE2
Plasmid#52271PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInserthTDG (TDG Human)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
mycUb-25aa
Plasmid#20256DepositorInsertUbiquitin-25 amino acid extension (RBX1 Human)
UseTagsmycExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf72
Plasmid#12760PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertA34R
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
hPGK-mRuby2-bGH
Plasmid#235254PurposeComMAND base gene regulating mRuby2, hPGK-drivenDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhPGK (human phosphoglycerate kinase)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp61(I342G)
Plasmid#228208PurposeExpression in BL21(DE3) of T4 primase I342GDepositorInsert61 (61 Escherichia phage T4)
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated isoleucine 342 to glycine (I342G), improv…PromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLANT-2b
Plasmid#231487PurposeExpresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.DepositorInsertsUseTagsHis6 tag and His6-SUMO^NITExpressionBacterialMutationDeleted amino acids 1-6 (MEPEES). and M83L, E84Q,…PromoterT7 promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM
Plasmid#225061PurposeExpression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225063PurposeExpression of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only