We narrowed to 12,431 results for: NSI
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Plasmid#92371PurposeInducible expression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterTet-responsive P tightAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch5_155183064-155183141
Plasmid#81211PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12-o11
Plasmid#69941PurposepSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanRDepositorInsertTheoHHAzRaj12,cisRaj11,sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12-olsa
Plasmid#69940PurposepSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanRDepositorInsertsTheoHHAzRaj12,cisRaj12,sfGFP
theoHHAzRaj12,cisRaj12-sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
refGFP1_pQE30
Plasmid#48644PurposeBact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFPDepositorInsertrefGFP1
Tags6xHisExpressionBacterialMutationrefGFP C147S, C204QAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-151)
Plasmid#37308DepositorAvailable SinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
mycUb-20aa
Plasmid#20255DepositorInsertUbiquitin-20 amino acid extension (RBX1 Human)
TagsmycExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …Available SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) Myc-tag-pSP72
Plasmid#8682DepositorInsertHOXB13 (HOXB13 Human)
UseTnt in vitroTagsMycExpressionBacterialMutationnew NsiI site at ATG startAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
TagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
YAP/TAZ-TRE-mStrawberry reporter
Plasmid#158682PurposeThis plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-Hyg-CTCF-N1
Plasmid#131799PurposeExpresses human CTCF-GFP in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
YAP/TAZ tdTomato TEAD Binding Reporter
Plasmid#228554PurposeEncodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.DepositorInserttdTomato
UseLentiviralExpressionMammalianPromoterGTIIC TEAD-Binding Responsive ElementAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
TagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
ExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBOB-HA-Gnaq
Plasmid#246001PurposeExpress HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
ExpressionMammalianMutationD210N, catalytically inactiveAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2-para-aminoPhe
Plasmid#85503PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only