19,771 results

Showing: 1651 - 1680 of 19771 results

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pSMT3-M

Plasmid

#26589

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

n/a

Use

Shuttle vector for mycobacteria

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A

Plasmid

#28047

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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AAV2/9 REP-AAP-ΔCAP

Plasmid

#205991

Purpose

Rep and AAP protein expression for AAV CREATE libraries

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

AAV9 Rep and AAP proteins for CREATE libraries

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG1_M18

Plasmid

#82357

Purpose

Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

hEF1-HTLV prom

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pICH41331::pU6cm-pegR::EURb7

Plasmid

#227695

Purpose

Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

Altered epegRNA backbone with cloning sites for gRNA and RTT.

Use

CRISPR

Expression

Plant

Promoter

U6 composite

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Promoter

human synapsin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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BirA pLEMO

Plasmid

#119818

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Expression

Bacterial

Promoter

Rhamnose promoter

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-PIP-FUCCI

Plasmid

#138715

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR

Plasmid

#28235

Purpose

Mammalian expression of Androgen receptor fused to EGFP

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

Androgen receptor (AR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-CHMP2B

Plasmid

#115329

Purpose

expresses mEGFP-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDestTol2CG2-U6:gRNA

Plasmid

#63156

Purpose

Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-2xFlag

Plasmid

#102929

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112005

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.661

Plasmid

#108532

Purpose

TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TtgRAM + PTtg-YFP

Expression

Bacterial

Mutation

TtgR: R130H A144T A148V

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pST39

Plasmid

#64009

Purpose

original polycistronic cloning vector for plasmid suite (generation I)

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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DT480

Plasmid

#80413

Purpose

Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti PGK GFP Puro (w509-5)

Plasmid

#19070

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBE14

Plasmid

#174653

Purpose

In vivo Cre-lox circularization in E. coli

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

Cre

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD-APEX2

Plasmid

#154939

Purpose

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Tags

V5

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP1-10

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pH2B-emiRFP670

Plasmid

#136571

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

H2B-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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19,771 results