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Showing: 2201 - 2240 of 4364 results

  11. ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187457
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187457-plasmid-map-sequence-id-367465

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  15. pJET-col2a1a-KI-donor

    Plasmid
    #184876
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184876-plasmid-map-sequence-id-362292

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  20. pCAG1.1-Armc12/FLAG

    Plasmid
    #180494
    Purpose
    Expression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
    Depositor
    Masahito Ikawa
    Article
    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118
    Insert
    armadillo repeat containing 12 (Armc12 Mouse)
    Use
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180494-plasmid-map-sequence-id-356913

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  25. pLCHKO_PDPR_exon_deletion_2

    Plasmid
    #155066
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155066-plasmid-map-sequence-id-308590

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  26. pLCHKO_SFRS7_exon_deletion_1

    Plasmid
    #155069
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155069-plasmid-map-sequence-id-308589

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  27. pLCHKO_SFRS7_exon_deletion_2

    Plasmid
    #155070
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155070-plasmid-map-sequence-id-308588

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  28. pLCHKO_MDM4_exon_deletion_1

    Plasmid
    #155073
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155073-plasmid-map-sequence-id-308586

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  29. pLCHKO_MDM4_exon_deletion_2

    Plasmid
    #155074
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155074-plasmid-map-sequence-id-308585

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  30. pLCHKO_MDM4_exon_deletion_4

    Plasmid
    #155076
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155076-plasmid-map-sequence-id-308577

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  31. pLCHKO_Ptbp_ex8_1

    Plasmid
    #155081
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155081-plasmid-map-sequence-id-308533

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  32. pLCHKO_Ptbp_ex8_4

    Plasmid
    #155084
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155084-plasmid-map-sequence-id-308527

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  33. pLCHKO_SFRS7_exon_deletion_4

    Plasmid
    #155072
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155072-plasmid-map-sequence-id-308512

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  34. pLCHKO_Ptbp_ex8_2

    Plasmid
    #155082
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155082-plasmid-map-sequence-id-308510

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  35. pLCHKO_PDPR_exon_deletion_3

    Plasmid
    #155067
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155067-plasmid-map-sequence-id-308446

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  36. pLCHKO_PDPR_exon_deletion_4

    Plasmid
    #155068
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155068-plasmid-map-sequence-id-308444

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  37. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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  38. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  39. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136653-plasmid-map-sequence-id-273329

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Showing: 2201 - 2240 of 4364 results