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Showing: 2751 - 2800 of 2952 results

  3. ZJOM136

    Plasmid
    #133660
    Purpose
    Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133660-plasmid-map-sequence-id-265888

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  4. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  11. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  14. EC71167

    Plasmid
    #154066
    Purpose
    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    All BsaI, Esp3I and BPiI restriction sites were r…
    Promoter
    Zea mays Ubiquitin Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154066-plasmid-map-sequence-id-312954

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  16. pRS405 SEC66-VAC8-RFP

    Plasmid
    #166843
    Purpose
    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Use
    Tags
    RFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166843-plasmid-map-sequence-id-329331

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  25. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  28. XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

    Plasmid
    #233382
    Purpose
    Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    VEGFR1(ecto+TM)-ADAR2dd
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233382-plasmid-map-sequence-id-466380

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  29. XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

    Plasmid
    #233386
    Purpose
    Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233386-plasmid-map-sequence-id-466377

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  31. mEos3.2trLATo

    Plasmid
    #203746
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATo (Lat Mouse)
    Use
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203746-plasmid-map-sequence-id-403345

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  33. pRS405 SEC66-VAC8-GFP

    Plasmid
    #166840
    Purpose
    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Use
    Tags
    GFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166840-plasmid-map-sequence-id-329328

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  40. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid
    #26966
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    ER export at 3' end of gene. Trafficking sig…
    Promoter
    EF1alpha
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26966-plasmid-map-sequence-id-200278

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  49. pHES839

    Plasmid
    #87941
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 (crippled)
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87941-plasmid-map-sequence-id-180360

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Showing: 2751 - 2800 of 2952 results