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Showing: 2751 - 2800 of 13556 results

  2. pCMV MN (eGFP)

    Plasmid
    #89932
    Purpose
    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    MN
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    M.SssI residues 2-272 fragment
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89932-plasmid-map-sequence-id-177997

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  6. pAC1750

    Plasmid
    #127984
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127984-plasmid-map-sequence-id-254510

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  8. pCS2+/N-SNAPf

    Plasmid
    #184415
    Purpose
    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184415-plasmid-map-sequence-id-365684

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  9. pCS2+/C-SNAPf

    Plasmid
    #184416
    Purpose
    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184416-plasmid-map-sequence-id-362158

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  11. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  37. pAC1748

    Plasmid
    #127982
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    127982-plasmid-map-sequence-id-254497

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Showing: 2751 - 2800 of 13556 results