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We narrowed to 75,184 results for: rest

Showing: 2801 - 2850 of 75184 results
  1. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid
    #181858
    Purpose
    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Insert
    FluoSTEP-EKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid
    #82469
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
    Depositor
    Insert
    mRNA capping enzyme (Rngtt Mouse)
    Use
    Tags
    Flag and Myc
    Expression
    Mammalian
    Mutation
    changed Lysine 294 to Alanine
    Promoter
    CMV
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pP[RS3]3'M

    Plasmid
    #53552
    Purpose
    for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila
    Depositor
    Insert
    AscI and MluI restriction enzyme sites
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    same pP[RS3]
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pENTR4 HopU1

    Plasmid
    #228395
    Purpose
    pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags
    Insert
    HopU1
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Alanine inserted after start Methionine due to Nc…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    Promoter
    P-LlacO-1
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pEPQD0KN0751

    Plasmid
    #185628
    Purpose
    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI
    Depositor
    Insert
    lacZ
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. TMD18

    Plasmid
    #163673
    Purpose
    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
    Depositor
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. TMD19

    Plasmid
    #163674
    Purpose
    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
    Depositor
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. FKBP-ST*-GFP

    Plasmid
    #163669
    Purpose
    Expresses FKBP tagged partial length ST in mammalian cells
    Depositor
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Use
    Tags
    FK506 binding protein (FKBP) and GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

    Plasmid
    #127776
    Purpose
    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus
    Insert
    tfap2b (TFAP2B Chicken)
    Use
    Unspecified
    Tags
    Avi
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. CC1

    Plasmid
    #63713
    Purpose
    Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain
    Depositor
    Insert
    CC1
    Use
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  15. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid
    #121966
    Purpose
    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
    Insert
    scFv-GCN4
    Use
    Lentiviral
    Tags
    superfold GFP-GB1-NLS-iLID
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. pcDNA 3.3 HA-hTSPAN12

    Plasmid
    #115784
    Purpose
    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.
    Depositor
    Insert
    TSPAN12 (TSPAN12 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    multiple silent mutations introduce restriction s…
    Promoter
    CMV
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 2801 - 2850 of 75184 results