We narrowed to 3,429 results for: Agt

1. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197958

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCVp and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #189946

   Purpose

   Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg3-Serpinh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Insert

   NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ5004_hU6_sgRNA targeting e4

   Plasmid

   #190686

   Purpose

   sgRNA targeting enhancer 4 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 4 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-sgRNA-Cas9.dora_1

   Plasmid

   #190700

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP4D41

   Plasmid

   #185841

   Purpose

   Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP10CF72 (B11)

   Plasmid

   #185859

   Purpose

   Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFP8EFA

   Plasmid

   #185854

   Purpose

   Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   DHFR P66L

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. rtTA-sg-tdTomato90/816

    Plasmid

    #188487

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFAP4_BHLH_5-1

    Plasmid

    #185054

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_5_1_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB9344 (pgRNA_XVI-1_NatMX)

    Plasmid

    #161596

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

    Plasmid

    #179007

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

    Plasmid

    #179001

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

    Plasmid

    #179000

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

    Plasmid

    #178998

    Purpose

    Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid

    #178095

    Purpose

    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EGFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSIN-Tpi1

    Plasmid

    #175940

    Purpose

    knockout mouse Tpi1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Tpi1 gRNA (Tpi1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-whiteCopyCatcher

    Plasmid

    #174062

    Purpose

    CopyCatcher insertion donor for the white locus

    Depositor

    Ethan Bier

    Article

    Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

    Insert

    white (w Synthetic, Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-GFP-mirNega

    Plasmid

    #163705

    Purpose

    pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

    Depositor

    Michisuke Yuzaki

    Article

    Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

    Insert

    mirNega and EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiCRISPR-v1-sgNADK_1

    Plasmid

    #163462

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting NADK

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 1 targeting NADK (NADK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

    Plasmid

    #149591

    Purpose

    all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRS424-ysg(G:H)

    Plasmid

    #138257

    Purpose

    Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    GFP targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19-hsg(G:H)

    Plasmid

    #138259

    Purpose

    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mCherry targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PX330_MAF1_iso1_2

    Plasmid

    #135750

    Purpose

    Encodes gRNA for 3' target of human MAF1_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MAF1_iso1 gRNA (MAF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gh89

    Plasmid

    #106760

    Purpose

    expression of gRNA targeting FUT5

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT5 (FUT5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. gh249

    Plasmid

    #106856

    Purpose

    expression of gRNA targeting PIGV

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    PIGV (PIGV Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gh104

    Plasmid

    #106774

    Purpose

    expression of gRNA targeting XXYLT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    XXYLT1 (XXYLT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAPM-D4 miR30-SUV39h2 ts1

    Plasmid

    #115883

    Purpose

    SUV39h2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h2 shRNA (SUV39H2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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