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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 160,082 results for: DES

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Showing: 11721 - 11740 of 160082 results

  6. pVP294

    Plasmid
    #222063
    Purpose
    GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    Backbone Esp3i sites have been domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222063-plasmid-map-sequence-id-447319

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  8. pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A

    Plasmid
    #82176
    Purpose
    Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection. (Please note this plasmid contains S45A mutation, not T45A)
    Depositor
    Jesse Boehm, William Hahn, David Root
    Article
    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi
    Insert
    CTNNB1 (CTNNB1 Human)
    Use
    Gateway entry vector
    Mutation
    S33A, S37A, T41A, S45A
    Promoter
    None
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82176-plasmid-map-sequence-id-163897

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  9. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

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  13. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

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  18. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

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Showing: 11721 - 11740 of 160082 results