59,428 results

1. pET28a_TIA1

   Plasmid

   #106095

   Purpose

   bacterial protein expression of TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   TIA1 (TIA1 Human)
   

   Tags

   6xHIs-thrombin and T7

   Expression

   Bacterial

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+_hsTRPA1_myc_dTom

   Plasmid

   #183179

   Purpose

   myc tagged hsTRPA1 used for cell transfection

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Inserts

   hsTRPA1 (TRPA1 Human)
   dTom
   

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Alpha-Actinin2-N-18

   Plasmid

   #54974

   Purpose

   Localization: Alpha Actinin, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Alpha-Actinin2 (ACTN2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV4-ApoE4

   Plasmid

   #87087

   Purpose

   Expresses ApoE4 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E4 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN236_CRISPRcharm_Kv2

   Plasmid

   #220839

   Purpose

   CRISPRcharm Kv2 expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm Kv2
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLICS Mt-ER- Po Long P2A

   Plasmid

   #164117

   Purpose

   Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Long P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. DNA-PKcs T3950D

   Plasmid

   #83320

   Purpose

   DNA-PKcs T3950A

   Depositor

   Katheryn Meek

   Article

   Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Expression

   Mammalian

   Mutation

   Activation loop phosphorylation site 3950 substit…

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #46295

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   PSD95.FingR-eGFP-CCR5TC (DLG4 Human, Synthetic)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-EGFP-Tau P301L

    Plasmid

    #46908

    Purpose

    expresses EGFP tagged Tau P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau P301L (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6/V5-p53_R273H

    Plasmid

    #22934

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R273H Mutant

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid

    #174660

    Purpose

    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    miniXon cassette
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. RMCE wild type DNA-PKcs

    Plasmid

    #233911

    Purpose

    Low copy number provides expression of human DNA-PKcs

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    dna-pkcs cDNA (PRKDC Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. ARR3tk-eGFP/SV40-mCherry

    Plasmid

    #132360

    Purpose

    To measure transcriptional activity of Androgen Receptor

    Depositor

    Charles Sawyers

    Article

    Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

    Insert

    AR responsive elements (AR Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #201987

    Purpose

    expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase (PDGFRA Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Tags

    flag-halo

    Expression

    Mammalian

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29b-rhMOG

    Plasmid

    #225484

    Purpose

    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

    Depositor

    John Pak

    Article

    Wu et al J Immunol Methods. 2024 Oct 13:113764. d

    Insert

    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-29 (signal peptide) and 150…

    Promoter

    T7

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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