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pC0043-PspCas13b crRNA backbone

Plasmid

#103854

Purpose

For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has Service

Cloning Grade DNA

Insert

U6-BbsI-BbsI-PspCas13b DR-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Tau (wt) -VC

Plasmid

#87369

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Insert

MAPT (MAPT Human)

Tags

c-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2/5

Plasmid

#232922

Purpose

AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

Depositor

Melina Fan, Addgene Research Program

Insert

Rep2/Cap5

Use

AAV

Expression

Mammalian

Available Since

Sept. 17, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1 pegRNA1 with trimmed attP

Plasmid

#222346

Purpose

pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA1

Use

CRISPR

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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phMYT1L-N174

Plasmid

#66809

Purpose

2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Blast

Plasmid

#52962

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Has Service

Lentiviral Prep

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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v5 Cas9-eVLP

Plasmid

#228468

Purpose

Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–Cas9

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

Plasmid

#134867

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCBASceI

Plasmid

#26477

Purpose

I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

Depositor

Maria Jasin

Article

Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

Insert

pCBASceI

Tags

HA

Expression

Mammalian

Available Since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pYFP-RPB1aAmr

Plasmid

#75284

Purpose

Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

Depositor

Xavier Darzacq, Robert Singer

Article

Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

Insert

POLR2A (POLR2A Human)

Tags

EYFP

Expression

Mammalian

Mutation

N792D alpha-amanitin–resistant

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-CreERT2

Plasmid

#14797

Purpose

Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pTight-9-124-BclxL

Plasmid

#60857

Purpose

2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Full length.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT1

Plasmid

#36939

Purpose

Mammalian expression of human DNMT1 with myc tag

Depositor

Arthur Riggs

Article

Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

Insert

DNMT1 (DNMT1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tdTomato (codon diversified)

Plasmid

#59462

Purpose

AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

Depositor

Edward Boyden

Has Service

AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

tdTomato

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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