165,971 results

1. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. OPA1-isoform1

   Plasmid

   #70173

   Purpose

   Mammalian expression of hsOPA1 isoform 1

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   hsOPA1 isoform 1 (OPA1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEP4 E02S EM2K

   Plasmid

   #20923

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
   

   Use

   Episomal/ebna

   Available Since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRISPRoff-mScarletI

   Plasmid

   #217783

   Purpose

   Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107307

   Purpose

   Encoding an SPH activation system

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ECFP HO

   Plasmid

   #207413

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ECFP CD

   Plasmid

   #207414

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-GFP-Dub3 mouse

   Plasmid

   #72683

   Purpose

   mammalian expression of GFP-Dub3

   Depositor

   Domenico Maiorano

   Article

   van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

   Insert

   Dub3 (Usp17le Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX307-FURIN-puro

   Plasmid

   #158460

   Purpose

   Constitutive lentiviral expression of human FURIN.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   FURIN (FURIN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-mCherry

    Plasmid

    #180646

    Purpose

    Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRPM4

    Plasmid

    #25903

    Depositor

    Craig Montell

    Article

    Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

    Insert

    TRPM4 (TRPM4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKD1-F (pCI-PKD1-Flag)

    Plasmid

    #21369

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECE-M2-PAK2 wt

    Plasmid

    #31663

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSum-LacZ-gfp

    Plasmid

    #109285

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GFP
    

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    LacZalpha

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-mCherry-NLS

    Plasmid

    #165442

    Purpose

    Expresses nuclear mCherry from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mCherry-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HSPB1

    Plasmid

    #155670

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    HSPB1 (HSPB1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid

    #113862

    Purpose

    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIB166

    Plasmid

    #90189

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Tags

    None

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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