We narrowed to 9,006 results for: Pol

1. FLAG-Pol-IIΔ

   Plasmid

   #35176

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the entire C-terminal domain (repeats…

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3basic luciferase and renilla_polyA

   Plasmid

   #128046

   Purpose

   pGL3 basic luciferase reporter with co-expression of renilla luciferase

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.GFAP.SV40.eGFP.SV40(polyA)

   Plasmid

   #195558

   Purpose

   Expresses eGFP under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   No tags

   Expression

   Mammalian

   Mutation

   Promoter

   short GFAP

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Use

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. gag-MCP-pol

   Plasmid

   #211370

   Purpose

   Packaging plasmid for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-MCP-pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-polyQ23-NLS

    Plasmid

    #231891

    Purpose

    Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q23-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOpen-GBDpol (exo)

    Plasmid

    #165572

    Purpose

    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mammalian Pol I

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSyn-BiPOLES-mCerulean

    Plasmid

    #154944

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-HA_WT POLK

    Plasmid

    #201584

    Purpose

    Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CaMKII-somBiPOLES-mCerulean

    Plasmid

    #154948

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-K12polLF (exo-)

    Plasmid

    #165522

    Purpose

    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pIRESpuro-Flag-Pol b

    Plasmid

    #23257

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

    Insert

    DNA polymerase beta (POLB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

21. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. p3e UbB polyA

    Plasmid

    #188702

    Purpose

    Gateway 3' element encoding the ubiquitin B polyadenylation sequence

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    UbB polyA (ubb Zebrafish)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. POLR2A-N CRISPR pX330

    Plasmid

    #124495

    Purpose

    A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A targeting CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. HO-pGAL-poly-KanMX4-HO

    Plasmid

    #51664

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA-HIRA-polyA

    Plasmid

    #59781

    Purpose

    Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. mDlx-BiPOLES-mCerulean

    Plasmid

    #154946

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal Dlx

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pVENUS-N1-POLR2G-GFP

    Plasmid

    #86872

    Purpose

    To express POLR2G (RPB7) tagged with VENUS at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    POLR2G (POLR2G Human)
    

    Use

    Tags

    VENUS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pOT2-poly-cis

    Plasmid

    #141177

    Purpose

    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

    Depositor

    Guiliang Tang

    Type

    Empty backbone

    Use

    RNAi and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. HO-pMET-poly-KanMX4-HO

    Plasmid

    #51665

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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