We narrowed to 325 results for: staphylococcus

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pTH103

Plasmid

#84461

Purpose

pJB38-NWMN29-30 + SarA_P1-YFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-YFP from pTH3

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRN110

Plasmid

#84462

Purpose

pJB38-NWMN29-30 + SarA_P1-mKate2-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mKate2 from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_9 [pUB110 ORI]

Plasmid

#198924

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGBMCS-SortA

Plasmid

#21931

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase (srtA Staphylococcus aureus (strain N315))

Tags

GB1 and His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only
pTH101

Plasmid

#84459

Purpose

pJB38-NWMN29-30 + SarA_P1-DsRed-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-DsRED from pTH1

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18/promSau/Lysostaphin

Plasmid

#125766

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK constitutive promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseB-6xHis

Plasmid

#214809

Purpose

Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease B

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGBHPS-SortA

Plasmid

#21930

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase

Tags

GB1, HRV3C cleagage site, and His

Expression

Bacterial

Mutation

deleted amino acids 1-59

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID

Plasmid

#188653

Purpose

Expresses SaAID in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nSaCas9-AID-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

pCMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD010

Plasmid

#116851

Purpose

Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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SAPDF-pET21a(+)

Plasmid

#12649

Depositor

SeWon Suh

Article

Yoon et al Proteins. 2004 Nov 15. 57(3):639-42.

Insert

SA Peptide deformylase

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGLU_64

Plasmid

#225767

Purpose

This is the native promoter from the ermC erythromycin resistance cassette.

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

PermC_[abr]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0012

Plasmid

#117533

Purpose

Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

eSaCas9 (no stop codon)

Use

CRISPR

Mutation

R499A,Q500K,R654A,G655R

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-pAG-TM

Plasmid

#207318

Purpose

Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

protein AG

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCN38-kimA-yfp

Plasmid

#227635

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Tags

6xHis (on sfGFP)

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRN11

Plasmid

#84455

Purpose

shuttle plasmid for sarA P1-mCherry expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mCherry

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRN112

Plasmid

#84464

Purpose

pJB38-NWMN29-30+ SarA_P1-mAmetrine-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mAmetrine from pRN12

Use

Synthetic Biology

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS9counter

Plasmid

#107192

Purpose

Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

Cas9
sgRNA

Expression

Bacterial

Promoter

prab17

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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