We narrowed to 818 results for: VILL;
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Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
TtGNN-GFP-empty
Plasmid#106380Purposedox inducible expression of GFP with neo resistanceDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCas9
Plasmid#41815PurposeExpresses human codon optimized Cas9 nuclease for genome engineeringDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOO2-GW
Plasmid#135097PurposeGateway vector for expression of proteins in Xenopus oocytesDepositorTypeEmpty backboneUseBacterial expression vector for generating sp6 tr…TagsExpressionMutationPromoterSP6Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo19
Plasmid#127612PurposeGFP-tagged Myosin-19DepositorInsertMyo19
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T1
Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T2
Plasmid#41820PurposeExpresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 WT
Plasmid#195213PurposeMammalian expression vector for GFP tagged NHERF1.DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
BFP-Rab5CA (Q79L)
Plasmid#205705PurposeMammalian expression of BFP-tagged mutant Rab5CADepositorInsertRAB5A (RAB5A Human)
UseTagsExpressionMammalianMutationQ79LPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4um200-ns
Plasmid#67696Purposemiddle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4um200
UseTagsHAExpressionMutationum200, no stop codon; N1056SPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
ciCas9_pcDNA5
Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-3H-GFP
Plasmid#205725PurposeExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundleDepositorInsertpOTC-3H-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPRL3_1-169
Plasmid#209796PurposeBacterial expression of human PRL-3 without farnesylation motifDepositorInsertPRL-3 (PTP4A3 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
786 pCSmAKT2Acherry
Plasmid#31167DepositorInsertAkt
UseExpression vectorTagsMyr and mCherryExpressionMutationmembrane tethered AktPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pME-6MTzactMap2k2b
Plasmid#67710PurposeMiddle entry cloneDepositorInsertactivated Map2k2b (map2k2b Zebrafish)
UseTagsExpressionMutationS221D, S225DPromoternoneAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKJ458
Plasmid#228709PurposeMammalian expression of Antibody C143 HC R39K (heavy chain); to be used with pKJ460 (Plasmid 228710) to make the antibodyDepositorArticleInsertC143 HC R39K
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ460
Plasmid#228710PurposeMammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibodyDepositorArticleInsertC143 LC N28R
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ423
Plasmid#228708PurposeMammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibodyDepositorArticleInsertAnti-CD71 LC
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisTagsExpressionYeastMutationPromoteryeast CYC-GAL and T7 promoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-GFP
Plasmid#205724PurposeExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylaseDepositorInsertpOTC-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEPT1
Plasmid#195001PurposeProduction of peptides in E. coli as GB1 fusionDepositorTypeEmpty backboneUseTagsHis8-tag and StrepTagII-GB1ExpressionBacterialMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GVcl 1-1066 /*TBM
Plasmid#162781Purposeamplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain IIDepositorArticleInsertGallus gallus vinculin (VCL Chicken)
UseTagsExpressionBacterialMutationchanged Gly 454 to ValPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMX1*_sgRNA
Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
UseTagsExpressionMutationPromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4-ns
Plasmid#67695Purposemiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4
UseTagsExpressionMutationno stop codon; D1302E (see depositor's comme…PromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-GFP
Plasmid#171123PurposeDoxycycline inducible expression of GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL-p27UTR
Plasmid#20881DepositorInsertp27 3'UTR (Cdkn1b Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-luciferase-P2A-Neo
Plasmid#105621Purpose3rd generation lentiviral plasmid for expression of luciferase with Neo resistanceDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRCherry2.1
Plasmid#108099PurposeLentiviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE_puro
Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo
Plasmid#108101PurposeLentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsFLAG tagExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:mNeonGreen-Rab5a
Plasmid#192355PurposezfRabaa tagged with mNeonGreen, expressed under the UAS promoterDepositorInsertRab5a (rab5aa Zebrafish)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDY1314_RADARS_Luciferase
Plasmid#193191PurposeExpress Gaussia Luciferase conditional upon RNA transcriptDepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDY1243-Fluorescent RADARS
Plasmid#182540PurposeExpress Fluorescent RADARS in bicistronic vector with mCherry as control geneDepositorInsertmNeon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-NEDD8
Plasmid#19943DepositorInsertNEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-GFP
Plasmid#114012PurposeEmpty sgRNA expression vector with co-expression of TurboGFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA_MS2_neo
Plasmid#118650PurposeTo clone sgRNA for activation dCas9DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDY1461_SERPINA1_CCA32_5avidity Cre
Plasmid#193193PurposeExpress Cre conditional upon RNA transcriptDepositorInsertCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MS2_P65_HSF1_2A_puro
Plasmid#118699PurposeTo express MS2-P65-HSF1DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab2WT
Plasmid#46779Purposeexpression of WT Rab2a in mammalian cellsDepositorInsertRAB2 (RAB2A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only