We narrowed to 13,711 results for: Cas9

1. Nme2Cas9_pCDest

   Plasmid

   #119923

   Purpose

   Nme2Cas9 CMV-driven mammalian expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGW_RUBY_EggCas9

   Plasmid

   #225990

   Purpose

   To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SlugCas9

   Plasmid

   #163793

   Purpose

   Expresses SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. hCas9_D10A

   Plasmid

   #41816

   Purpose

   Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9_D10A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A nickase

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sa-SlugCas9

   Plasmid

   #163797

   Purpose

   Expresses Sa-SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SlutrCas9

   Plasmid

   #163795

   Purpose

   Expresses SlutrCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSSV_LTR

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiV_Cas9_Blast

    Plasmid

    #125592

    Purpose

    Lentiviral expression of spCas9 with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pScCas9

    Plasmid

    #117700

    Purpose

    Mammalian expression vector encoding WT ScCas9

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

    Insert

    Streptococcus canis Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBR_Cas9_sp1

    Plasmid

    #154851

    Purpose

    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

    Depositor

    John van der Oost

    Article

    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

    Inserts

    Spcas9 gene
    sgRNA scaffold
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Mutation

    Codon harmonized variant for expression in Rhodob…

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS42H_PTEF1_dCas9_TCYC1

    Plasmid

    #177155

    Purpose

    Expresses dCas9 in yeast cells

    Depositor

    Gautam Dantas

    Article

    Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

    Insert

    Nuclease-null Cas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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