We narrowed to 16,745 results for: EMB

1. pcDNA3-pmEKAR4

   Plasmid

   #174440

   Purpose

   Plasma membrane-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   pmEKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Perox-RA

   Plasmid

   #209869

   Purpose

   Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Use

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMM2-hPMCA4b

   Plasmid

   #47759

   Purpose

   mammalian expression of human PMCA4b

   Depositor

   Emanuel Strehler, John Penniston

   Article

   Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

   Insert

   PMCA4b (ATP2B4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TC1681

   Plasmid

   #164872

   Purpose

   CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

   Plasmid

   #203229

   Purpose

   Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

   Insert

   Cre-dependent, membrane-localized Optopatch
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES:hTRAAK

   Plasmid

   #133080

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full length

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TC1614

   Plasmid

   #164873

   Purpose

   CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A inserted into RfxCas13d (APOBEC3A )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC_C_TYRO3

   Plasmid

   #187793

   Purpose

   MAC-tagged gene expression of human TYRO3

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   TYRO3 (TYRO3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-MSP1D1deltaH4H5

    Plasmid

    #71716

    Purpose

    Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS2142

    Plasmid

    #184273

    Purpose

    Tet-off genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

    Insert

    CatTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. myc-hIFITM3-delta59-68

    Plasmid

    #104361

    Purpose

    Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deleted amino acids 59 through 68

    Promoter

    CMV

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ubiquitin (1-76; T66V, L67N)

    Plasmid

    #110755

    Purpose

    Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T66V, L67N

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1E2

    Plasmid

    #20063

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E2 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV126

    Plasmid

    #63192

    Purpose

    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBTK107

    Plasmid

    #110575

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP25 Promoter with RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCS2-BiNLS-fYap

    Plasmid

    #176623

    Purpose

    expresses optofYap in zebrafish

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    Yes associated protein (yap1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mbYFPQS

    Plasmid

    #80742

    Purpose

    membrane-targeted biosensor for monitoring changes in intracellular chloride

    Depositor

    Susan Amara, Susan Ingram

    Article

    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

    Insert

    YFPQS
    

    Use

    Tags

    palmitoylation signal sequence of neuromodulin

    Expression

    Mammalian

    Mutation

    H148Q (CAC to CAG) and V163S (GTG to AGC).

    Promoter

    CMV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSP1E1

    Plasmid

    #20062

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeat of hel…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOPS0254

    Plasmid

    #115506

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Perox-GA

    Plasmid

    #209868

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSCIP-mB2M

    Plasmid

    #154091

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting murine B2M

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MAC_C_EphA7

    Plasmid

    #187765

    Purpose

    MAC-tagged gene expression of human EphA7

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EphA7 (EPHA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.hSyn.Cre

    Plasmid

    #170367

    Purpose

    AAV vector expressing Cre under human synapsin promotor

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAV125

    Plasmid

    #63191

    Purpose

    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-MSP1D1delltaH4

    Plasmid

    #71713

    Purpose

    Membrane scaffold protein (MSP), helix 4 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCer-4aa-VAMP2

    Plasmid

    #53233

    Purpose

    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pMV_AA089

    Plasmid

    #216105

    Purpose

    gRNA expression for Cas12a (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gRNA expression
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MAC_C_EPHB2

    Plasmid

    #187797

    Purpose

    MAC-tagged gene expression of human EPHB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB2 (EPHB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MAC_C_MUSK

    Plasmid

    #187781

    Purpose

    MAC-tagged gene expression of human MUSK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    MUSK (MUSK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-IRAlus-Apobec3G

    Plasmid

    #92349

    Purpose

    Plasmid for expression of GFP gene with a Apobec3G gene intron derived IR Alu element containing 3'UTR.

    Depositor

    Ling-Ling Chen, Gordon Carmichael

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Apobec3G gene intron derived IR Alu element containing 3'UTR (APOBEC3G Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMM2-hPMCA1b

    Plasmid

    #47758

    Purpose

    mammalian expression of human PMCA1b

    Depositor

    Emanuel Strehler, John Penniston

    Article

    Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

    Insert

    PMCA1 (ATP2B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-Alus-Apobec3G

    Plasmid

    #92348

    Purpose

    Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

    Depositor

    Ling-Ling Chen, Gordon Carmichael

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCEP4-hKCNE1

    Plasmid

    #53050

    Purpose

    Expresses beta subunit for IKs channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHAGE-UBC-TagRFP-T-ps

    Plasmid

    #178656

    Purpose

    Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

    Insert

    TagRFP-T with palmitoylation signal at N-terminus
    

    Use

    Lentiviral

    Tags

    Palmitoylation signal

    Expression

    Mutation

    Promoter

    Ubc

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBTK519

    Plasmid

    #110603

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge