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We narrowed to 15,192 results for: genome

Showing: 2361 - 2400 of 15192 results

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pBTK800

Plasmid

#179209

Purpose

GFP dropout for assembling a double-stranded RNA expression plasmid

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

GFP dropout

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Tags

Expression

Mutation

Promoter

vasa

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only
pKDR-Lenti-TetOn

Plasmid

#183555

Purpose

pKDR compatible TetOn lentiviral transfer plasmid

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_H2_beta_GM

Plasmid

#184871

Purpose

pSwap plasmid part with H2_beta homology arms and gentamycin cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p426*-SpSgH

Plasmid

#136993

Purpose

Helper plasmid for SpCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV5

Plasmid

#78629

Purpose

GESTALT V5 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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PL-452 C-EGFP

Plasmid

#19178

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

Insert

EGFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
p3-K230-FokI_L (wt)

Plasmid

#32544

Depositor

Jin-Soo Kim

Article

Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

Insert

ZFN

Use

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only
p3-K230-FokI_R (wt)

Plasmid

#32545

Depositor

Jin-Soo Kim

Article

Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

Insert

ZFN

Use

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only
pCFD4d

Plasmid

#83954

Purpose

Modified from pCFD4, with the vermillion marker and the attB site removed

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

dU6-1; dU6-3

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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MCV

Plasmid

#81174

Purpose

MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR-BluntII-TOPO-RhoC

Plasmid

#14099

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

Human RhoC (RHOC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
beta2 (px330:2-guideCas9)

Plasmid

#169435

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC151-3XUAS-IVS-Flp2::PEST

Plasmid

#32133

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Flippase2::PEST

Use

Tags

PEST

Expression

Insect

Mutation

Glycine at Amino Acid 5

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pJW1311

Plasmid

#61254

Purpose

Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting Y61A9L9.1

Use

CRISPR; Cloning template

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSWAP_Entry_HA

Plasmid

#184866

Purpose

pSwap plasmid part with HA homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSWAP_Entry_HB

Plasmid

#184868

Purpose

pSwap plasmid part with HB homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDD132

Plasmid

#119885

Purpose

Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

BbaJ23104_EYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-C3orf17-gfp

Plasmid

#70265

Purpose

Expresses C3orf17 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C3orf17 (NEPRO Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
GluA3 (px330:2-guideCas9)

Plasmid

#169424

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2038

Plasmid

#39396

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2039

Plasmid

#39397

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pET15b-pA-Mnase

Plasmid

#124883

Purpose

To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

Depositor

Keji Zhao

Article

Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

Insert

protein A-MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p426*-LbSgH

Plasmid

#136992

Purpose

Helper plasmid for LbCpf1 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pECT3

Plasmid

#75450

Purpose

Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt3

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF-LmrA

Plasmid

#49364

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LmrA repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PL-452 N-EGFP

Plasmid

#19173

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

Insert

EGFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-T3-hCASeGFP-pA

Plasmid

#59766

Purpose

Expresses CAS9-eGFP fusion protein in mammalian cells.

Depositor

Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

CAS9

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2084

Plasmid

#39452

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-48-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pSEVA83-GG

Plasmid

#208016

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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