We narrowed to 12,205 results for: 112

1. Venus-BimEL(Noxa)-pEGFP-C1

   Plasmid

   #166761

   Purpose

   To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   BimEL(Mus musculus)-(Noxa BH3) (Bcl2l11, PMAIP1 Human, Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   BH3 region removed and inserted BH3 of human Noxa…

   Promoter

   CMV

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. A1-LCD Aro--

   Plasmid

   #169716

   Purpose

   Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.

   Depositor

   Tanja Mittag

   Article

   Martin et al Science. 2020 Feb 7;367(6478):694-699. d

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hnRNPA1-A Low complexity domain including amino a…

   Promoter

   T7

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. A1-LCD Perfect Mix

   Plasmid

   #169718

   Purpose

   Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. TYR and PHE shuffled into 5 clusters.

   Depositor

   Tanja Mittag

   Article

   Martin et al Science. 2020 Feb 7;367(6478):694-699. d

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hnRNPA1-A Low complexity domain including amino a…

   Promoter

   T7

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. A1-LCD Aro-

   Plasmid

   #169715

   Purpose

   Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.

   Depositor

   Tanja Mittag

   Article

   Martin et al Science. 2020 Feb 7;367(6478):694-699. d

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hnRNPA1-A Low complexity domain including amino a…

   Promoter

   T7

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. A1-LCD Aro+

   Plasmid

   #169717

   Purpose

   Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.

   Depositor

   Tanja Mittag

   Article

   Martin et al Science. 2020 Feb 7;367(6478):694-699. d

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hnRNPA1-A Low complexity domain including amino a…

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. A1-LCD Patchy

   Plasmid

   #169719

   Purpose

   Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.

   Depositor

   Tanja Mittag

   Article

   Martin et al Science. 2020 Feb 7;367(6478):694-699. d

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hnRNPA1-A Low complexity domain including amino a…

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

   Plasmid

   #72924

   Purpose

   Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA Binding Protein 6 (GATA6 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3XAU1 and 3XFLAG

   Expression

   Bacterial

   Mutation

   Serine#37 changed to Alanine;Silent mutations mad…

   Promoter

   TRE tight

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

   Plasmid

   #72617

   Purpose

   Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA Binding Protein 6 (GATA6 Human)
   

   Use

   Lentiviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   Serine#37 changed to Alanine;Silent mutations mad…

   Promoter

   TRE tight

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CASK gRNA (BRDN0001145000)

   Plasmid

   #77402

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CASK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CASK (CASK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CASK gRNA (BRDN0001148068)

    Plasmid

    #77403

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CASK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CASK (CASK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CASK gRNA (BRDN0001149003)

    Plasmid

    #77404

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CASK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CASK (CASK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HRas (G12V, E37G)-pcw107

    Plasmid

    #64639

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    HRAS (transcript variant 3) (HRAS Human)
    

    Use

    Lentiviral

    Mutation

    G12V, E37G

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

13. HRas (G12V, E37G)-pcw107-V5

    Plasmid

    #64640

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    HRAS (transcript variant 3) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    G12V, E37G

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. G13-CASE

    Plasmid

    #168127

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F125/D126 within GNA13

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid

    #103871

    Purpose

    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SARS-CoV-2 S HexaPro

    Plasmid

    #154754

    Purpose

    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

    Depositor

    Jason McLellan

    Article

    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

    Insert

    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge