Addgene

We narrowed to 17,141 results for: puro

Showing: 2401 - 2440 of 17141 results

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBRY-nuclear mCherry-IRES-PURO

Plasmid

#52409

Purpose

Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

nuclear mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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1119 pBabe puroL HA FKHR AAA

Plasmid

#9025

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

T24A S256A S319A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

Plasmid

#191527

Purpose

Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

CRISPR and Synthetic Biology

Tags

mNeonGreen (mNG) + 3xFLAG_TwinStrep

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-2XFLAG,2XHA-CyclinF-puro

Plasmid

#32976

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE puro human RXR alpha

Plasmid

#11441

Depositor

Ronald Kahn

Insert

RXR alpha (RXRA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTR AA Clover

Plasmid

#90238

Purpose

Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AA mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-ROCK2-C (941-1388)

Plasmid

#225471

Purpose

Express mEGFP-fusion protein of ROCK2 fragment (941-1388)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

ROCK2 (ROCK2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

C terminal fragment (941-1388)

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-hSOX2-EGFP-PuroTK

Plasmid

#141026

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

Plasmid

#162098

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to HA-FLAG-AU1

Use

Lentiviral

Tags

HA-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V T35S

Plasmid

#12274

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(H129N)

Plasmid

#208394

Purpose

To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Tags

Expression

Mammalian

Mutation

H129N

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3-Puro

Plasmid

#172619

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 SOX17 g2

Plasmid

#155188

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d SOX17 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

Plasmid

#139445

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA284= pSin-Pur-MCS-Puro

Plasmid

#116877

Purpose

Lentiviral transfer vector containing a multible cloning site

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-TetOne-Puro-p62 deltaUBA

Plasmid

#204550

Purpose

Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

deltaUBA

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro U6 sgRNA SOX17 -177

Plasmid

#50925

Purpose

U6 driven sgRNA targeting Sox17 -177 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -177 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVSP6-NR2F2-SV40-PURO

Plasmid

#138361

Purpose

Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Human NR2F2, with puromycin driven by SV40 for selection

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lv EF1a HP1cs-Frb1x PGK Puro

Plasmid

#102808

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

HP1cs-Frb1x

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-HA-UBE3B(C1036A)-IRES-Puro

Plasmid

#107348

Purpose

Expresses HA tagged UBE3B mutant C1036A in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A) (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

changed Cysteine 1036 to Alanine

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Use

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

Plasmid

#163176

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT-CMV-UBE3B-copGFP-Puro

Plasmid

#107342

Purpose

Expresses UBE3B fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

Ubiquitin-protein ligase E3B (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

Plasmid

#192682

Purpose

Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Puro CAG IL13-CAR

Plasmid

#157742

Purpose

knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Chimeric antigen receptor targeting IL13

Use

CRISPR, Synthetic Biology, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
911 pBabe puroL PTEN G129E

Plasmid

#10771

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G129E

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-Flag-Polß(WT)-IRES-puro

Plasmid

#128653

Purpose

mammalian expression of Flag-Polß(WT) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-RAP1A-E63-puro

Plasmid

#156167

Purpose

Lentiviral vector for expression of RAP1A-E63, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC118-mCtnnb1-FLIP-FlpE-Puro

Plasmid

#84541

Purpose

CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP-FlpE - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Article

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Use

Tags

12X MS2 stem loops

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK Puro DEST JNKKTR EE Clover

Plasmid

#90239

Purpose

Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (EE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLC-Flag-CSNK1A1 G40N-Puro

Plasmid

#123320

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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