We narrowed to 9,252 results for: SUB
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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM
Plasmid#225061PurposeExpression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225063PurposeExpression of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Xlone Puro SNAP-eGFP-BASU
Plasmid#218452Purposeexpresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integrationDepositorInsertSNAP-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_NLS-Halo-eGFP-BASU
Plasmid#218449Purposeexpressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP
Plasmid#184819PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.DepositorInsertQF2-pA-he1.1:YFP
UseCRISPR; Backbone for cloning of homology arms (fo…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C1-IFT22
Plasmid#218720PurposeExpresses N-terminally EGFP-tagged IFT22 in mammalian cellsDepositorInsertIFT22 (RABL5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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SUMO CRYGS 16-177 Q16C
Plasmid#217673PurposeN-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligationDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to Cysteine, and amino acids…PromoterT7Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP8B4-HA
Plasmid#209229PurposeMammalian expression of ATP8B4DepositorInsertATP8B4 (ATP8B4 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP8B3-HA
Plasmid#209228PurposeMammalian expression of ATP8B3DepositorInsertATP8B3 (ATP8B3 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196E wgMDH
Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126R wgMDH
Plasmid#204261PurposeBacterial expression of P126R wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126RPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127A wgMDH
Plasmid#204263PurposeBacterial expression of G127A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127P wgMDH
Plasmid#204265PurposeBacterial expression of G127P wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127PPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121F wgMDH
Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126G wgMDH
Plasmid#204260PurposeBacterial expression of P126G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123G wgMDH
Plasmid#204256PurposeBacterial expression of P123G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123F wgMDH
Plasmid#204255PurposeBacterial expression of P123F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A D197,199N
Plasmid#195704PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutationsDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9 with D1…PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-TIS11B
Plasmid#207670PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11BDepositorInsertTIS11B (ZFP36L1 Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-BR1
Plasmid#192816PurposeExpresses BR1 in bacteriaDepositorInsertBR1 (BRI1 Mustard Weed)
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYG205
Plasmid#200836PurposeTranscribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivativesDepositorInsertrapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP-BADAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-RUNX3 aa 49-187
Plasmid#203440PurposeBacterial expression of RUNX5DepositorInsertRUNX3 (RUNX3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchCAST-crtYf
Plasmid#203816PurposeExpression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchDonor
Plasmid#203817PurposeDNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertMini-Tn, Spec cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-eGFP-SAPAP3
Plasmid#176853PurposeExpresses SAPAP3 protein fused with eGFP at the N-terminus in neuronsDepositorInserteGFP-SAPAP3
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only
