We narrowed to 6,480 results for: poly
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Plasmid#72971PurposeLevel 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM908 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM910
Plasmid#72972PurposeLevel 0 - SJM910 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM910 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM912
Plasmid#72974PurposeLevel 0 - SJM912 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM912 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM914
Plasmid#72975PurposeLevel 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM914 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM915
Plasmid#72976PurposeLevel 0 - SJM915 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM915 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7
Plasmid#72977PurposeT7 promoter (5' CTAT/3' GTAC fusion)DepositorInsertT7 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP_BBa_B0034
Plasmid#72980PurposeBba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)DepositorInsertBba_B0034 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-Tag_linker
Plasmid#72982PurposeN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)DepositorInsertN-terminal linker with pET-RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-HexHis
Plasmid#72983PurposeHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertPolyhistidine (HHHHHH) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-Strep(II)
Plasmid#72984PurposeStreptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertStrep(II) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL1
Plasmid#72985PurposeRBS_H1DepositorInsertH1 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL2
Plasmid#72986PurposeRBS_H2DepositorInsertH2 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL3
Plasmid#72987PurposeRBS_H3DepositorInsertH3 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-C-lacZ
Plasmid#72937PurposeLevel 1 Destination vector - Position CDepositorInsertLevel 1 - C site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-lacZ
Plasmid#72938PurposeLevel 1 Destination vector - Position DDepositorInsertLevel 1 - D site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-C-RFP
Plasmid#72941PurposeLevel 1 Destination vector - Position CDepositorInsertLevel 1 - C site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-RFP
Plasmid#72942PurposeLevel 1 Destination vector - Position DDepositorInsertLevel 1 - D site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D1-RFP
Plasmid#72943PurposeLevel 1 Destination vector - Position D1DepositorInsertLevel 1 - D1 site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-E-RFP
Plasmid#72944PurposeLevel 1 Destination vector - Position EDepositorInsertLevel 1 - E site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eGFP
Plasmid#72960PurposeLevel 0 Entry vector" ORF - eGFP "DepositorInsertEnhanced green flourescent protein (eGFP)
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RRN3
Plasmid#67112PurposeStandard for cell-free expression benchmarking.DepositorInsertRRN3 (RRN3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP4
Plasmid#67073PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAP4 (TFAP4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationP338APromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV2
Plasmid#180516PurposeAgrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinkerDepositorInsertTobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-GFPd2
Plasmid#115665PurposePiggybac transposon plasmid for destabilized eGFP (GFPd2)DepositorInsertGFPd2
UseSynthetic BiologyTagsDestabilizationExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC
Plasmid#64874Purposelentiviral vector with Flag, HA tagsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX333-T2A-EGFP
Plasmid#197417PurposeSpCas9-T2A-EGFP with dual sgRNA cloning backbonesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBh; U6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX
Plasmid#129285PurposeLentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequenceDepositorInsertFluorescent reporter mCherry fused to CAAX polybasic sequence
UseLentiviralTagsCAAXExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2BD
Plasmid#234428PurposePlasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified Cas9 with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mNFM
Plasmid#32909PurposeMammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein MDepositorInsertEGFP-mouse NFM fusion (Nefm Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_hTRIM32
Plasmid#69541PurposeExpresses human TRIM32 fused to c-terminal EGFPDepositorInserttripartite motif containing 32 (TRIM32 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
NylCK-TS_pET28a
Plasmid#215423PurposeExpression construct for NylCK-TS gene, codon optimized for expression in E. coliDepositorInsertNylCK-TS
UseTagsExpressionBacterialMutationS111G/A137LPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-pmDH
Plasmid#67897PurposeExpresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cellsDepositorInsertmCherry-pmDH
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEx-deletion-erm
Plasmid#172213PurposeFor allelic exchange in erythromycin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseAllelic exchange in prevotella copriTagsExpressionMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEx-deletion-tet
Plasmid#172214PurposeFor allelic exchange in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseAllelic exchange in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFC43
Plasmid#89488Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-C (aa 154-240)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Neo
Plasmid#125593PurposeLentiviral expression of sgRNA with GFP and neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFN43
Plasmid#89487Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-N (aa 1-154)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorInsertNLS-I-PpoI
UseTagsHAExpressionMammalianMutationPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
UseTagsExpressionBacterial and YeastMutationE366G (See depositor comment below)PromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA14-pHR-SFFV-GFP-RPA43
Plasmid#199445PurposeExpression of GFP-RPA43 in mammalian cellsDepositorInsertRPA43 (POLR1F Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AfNitB_pET29b
Plasmid#215401PurposeExpression construct for AfNitB gene, codon optimized for expression in E. coliDepositorInsertAfNitB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
UseTagsExpressionBacterialMutationS111G/A137L/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2-TS_pET28a
Plasmid#215421PurposeExpression construct for NylCp2-TS gene, codon optimized for expression in E. coliDepositorInsertNylCp2-TS
UseTagsExpressionBacterialMutationD122G/H130Y/D36A/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPM1003
Plasmid#167550PurposeEncodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12DepositorInsertVibrio cholerae O antigens genes of the Ogawa serotype
UseCosmidTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only