We narrowed to 59,686 results for: plasmid
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Plasmid#64125PurposePhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAL4UAS
UseLuciferaseTagsluciferaseExpressionMammalianPromoterGAL4UASAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PGC-1 alpha promoter luciferase delta CRE
Plasmid#8888DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPGC-1 alpha promoter dCRE (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationSite-directed mutagenesis to remove the CREB bind…Available sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GMutationS34C mutation in NefAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)
UseLentiviralAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0040-LwaCas13a crRNA backbone
Plasmid#103851PurposeBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-LwaCas13aDR-BbsI-BbsI-polyT
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
TagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322PurposeGateway entry vector containing ubi:loxP-EGFP-loxP. Used to assemble ubi:Switch construct.DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4 mIL-17 2kb promoter
Plasmid#20124DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmIL-17 (Il17a Mouse)
UseLuciferaseAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCathepsin L
Plasmid#11250PurposeMammalian expression of human cathepsin LDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcathepsin L (CTSL Human)
TagsC9 (GTETSQVAPA)ExpressionMammalianAvailable sinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pGEM-LIN28
Plasmid#16350DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
ExpressionBacterialAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TetO-FUW-Klf4
Plasmid#20322DepositorAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRE-mCherry
Plasmid#92202PurposeFluorescent protein reporter constructDepositorInsertmCherry
UseAAVExpressionMammalianPromoterTREAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)Available sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB_rtTA_BsmBI
Plasmid#126028PurposePiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloningDepositorTypeEmpty backboneUseCRISPR; PiggybacExpressionMammalianPromoterU6Available sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pCMV-15F11-HA-mCh
Plasmid#129591PurposeEncodes anti-HA scFv (anti-HA frankenbody) fused with mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
ExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by