8,807 results

1. ATeam1.03-nD/nA/pcDNA3

   Plasmid

   #51958

   Purpose

   mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

   Insert

   ATeam1.03-nD/nA
   

   Expression

   Mammalian

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-EF1a-Backbone(NG)

   Plasmid

   #27963

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TAL-Backbone(NG)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT040

   Plasmid

   #67640

   Purpose

   URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Insert

   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-hPIASx alpha

   Plasmid

   #15209

   Depositor

   Ke Shuai

   Article

   Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

   Insert

   PIASx alpha (PIAS2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

7. LT3GEPIR

   Plasmid

   #111177

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-SIRT1

   Plasmid

   #1791

   Purpose

   Mammalian expression of flag-tagged SIRT1

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdTrack-CMV

   Plasmid

   #16405

   Purpose

   Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV/TO SV40 small + Large T (w612-1)

    Plasmid

    #22298

    Depositor

    Eric Campeau

    Insert

    SV40 small + Large T antigen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-mPIAS3

    Plasmid

    #15207

    Depositor

    Ke Shuai

    Article

    Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

    Insert

    PIAS3 (Pias3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V580I

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Promoter

    CMV

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV/TO eGFP Puro (w159-1)

    Plasmid

    #17481

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xHA-miniTurbo-NLS_pCDNA3

    Plasmid

    #107172

    Purpose

    expresses 3xHA-tagged miniTurbo in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJR104

    Plasmid

    #187243

    Purpose

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Tags

    EE and GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C3-Sec5

    Plasmid

    #53756

    Purpose

    pEGFP-C3 plasmid that expresses Sec5

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec5 (EXOC2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only