20,039 results

Showing: 1261 - 1280 of 20039 results

Results per page:

Sort results by:

gag-COM-pol

Plasmid

#211373

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-COM-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

Plasmid

#139999

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

Promoter

CAG

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-DHX36iso1

Plasmid

#159585

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCC001

Plasmid

#124451

Purpose

Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HH-BsaI

Expression

Yeast

Mutation

gRNA expression cassette from pUDP002 modified to…

Promoter

TDH3 promoter from S.cerevisiae (already present …

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG vGlut1 mCherry

Plasmid

#105010

Purpose

Excitatory presynaptic marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-HSPA8

Plasmid

#121161

Purpose

Mammalian expression of GFP tagged HSPA8

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HSPA8 (HSPA8 Human)

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Alpha-Synuclein-His

Plasmid

#186802

Purpose

Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Alpha-Synuclein-His (SNCA Human)

Tags

His6

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

Plasmid

#113888

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Lifeact-tdTomato

Plasmid

#64048

Purpose

Fluorescent reporter for F-actin labeling in living cells

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Lifeact

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
psPax2-D64V-NC-mNeon

Plasmid

#196509

Purpose

mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

mNeon

Tags

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Mutation

L124T; K125-LA-Ins

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sfGFP-C1

Plasmid

#54579

Purpose

Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Article

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

sfGFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Tobias Meyer, Sabrina Spencer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Ub

Plasmid

#11928

Purpose

Used for in vivo monitoring of ubiquitin localization.

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (UBC Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-TurboID-NES_pCDNA3

Plasmid

#107169

Purpose

expresses V5-tagged TurboID in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM495 (Flag-Das+4) Zeo

Plasmid

#109284

Purpose

ORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-DAS+4

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge