161,488 results

1. FUG-T2A-cre

   Plasmid

   #66691

   Purpose

   Recombine floxed alleles in any cell

   Depositor

   Bryan Luikart

   Article

   Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

   Insert

   GFP-T2A-Cre
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221_SLC39A10

   Plasmid

   #132275

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A10 (SLC39A10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pET21a-TEX264_wt-His6

   Plasmid

   #220214

   Purpose

   Bacterial expression of wild type TEX264, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC30A3

   Plasmid

   #132101

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC30A3 (SLC30A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18T-mini-Tn7T-hph-Para

   Plasmid

   #218363

   Purpose

   Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p23-NES-PspCas13b-msfGFP-NES-Flag

   Plasmid

   #165071

   Purpose

   overexpression of PspCas13b in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDZ2FE_Champ1_1-812

   Plasmid

   #105675

   Purpose

   Expresses Flag-tagged full-length human Champ1 protein

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAT-PB-CG2APtk

   Plasmid

   #86003

   Purpose

   homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   eGFP_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2144

    Plasmid

    #104357

    Purpose

    AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

    Insert

    ssAAV-smCBA-EmGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NSP6-FR-2Strep

    Plasmid

    #210317

    Purpose

    Expresses SARS-CoV-2 NSP6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pM378

    Plasmid

    #173174

    Purpose

    Expresses C9-1 gRNA-12xMBS from hU6 promoter

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    C9-1 targeting gRNA-12xMBS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. keratin 8 (krt8)-H2B-RFP

    Plasmid

    #105955

    Purpose

    transgenesis

    Depositor

    Caren Norden

    Article

    Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

    Insert

    krt8 (KRT8 Human)
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CS2 Flag-Smad2 (3A)

    Plasmid

    #101763

    Purpose

    Expresses SMAD2 (3A) in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Three Ser to Ala mutations in the phosphorylation…

    Promoter

    CMV

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p23-NES-PguCas13b-msfGFP-NES-Flag

    Plasmid

    #165072

    Purpose

    overexpression of PguCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSSa30

    Plasmid

    #179502

    Purpose

    Tet-on red-shifted luciferase expression

    Depositor

    Dominique Sanglard

    Article

    Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

    Insert

    Red-shifted luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    TEF1t-HOP1 (p99CGH)

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. plex_307-ccdB-iRFP713

    Plasmid

    #201140

    Purpose

    pLEX_307 plasmid with iRFP713 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_GAS7-1/1

    Plasmid

    #91301

    Purpose

    Protein expression and purification of human SH3 domain construct GAS7-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GAS7-1/1 (GAS7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. R777-E189 Hs.PREX2

    Plasmid

    #70473

    Purpose

    Gateway ORF clone of human PREX2 [NM_024870.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PREX2 (PREX2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH41331

    Plasmid

    #47999

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge