166,169 results

1. pIG-703pUC19-tNGFR-P2A-BACH2

   Plasmid

   #186112

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BACH2 (BACH2 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR221_SLC4A5

   Plasmid

   #132284

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC4A5 (SLC4A5 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pTR-469 JUNB-tNGFR

   Plasmid

   #186060

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   JUNB (JUNB Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTR-472 FOXO1-tNGFR

   Plasmid

   #186062

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   FOXO1 (FOXO1 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pafG-CBE-3T

   Plasmid

   #220374

   Purpose

   for hA3A-nCas9 and gRNA expression in Aspergillus flavus

   Depositor

   Xue Gao

   Article

   Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

   Inserts

   hA3A-nCas9
   tRNA and gRNA scaffold
   

   Use

   CRISPR

   Promoter

   Ptef1 and tRNA

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tetO-ETS2

   Plasmid

   #170685

   Purpose

   doxycycline-inducible overexpression of ETS2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   ETS2 (ETS2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1_hSMAD4

   Plasmid

   #212710

   Purpose

   The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

   Depositor

   Catharine Ross, Reza Zolfaghari

   Insert

   Homo sapiens SMAD4 (SMAD4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEY17

   Plasmid

   #191023

   Purpose

   flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-18 CELE_Y48D7A.2 (flp-18 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-LacI-polylinker

    Plasmid

    #179271

    Purpose

    Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Type

    Empty backbone

    Tags

    GFP and LacI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPAP002

    Plasmid

    #153489

    Purpose

    Level 1 episomal expression plasmid for P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTR-471 IRF4-tNGFR

    Plasmid

    #186061

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IRF4 (IRF4 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid

    #117319

    Purpose

    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-CTRL
    

    Use

    Transposon-mediated integration

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    miR-124 deleted

    Promoter

    Tet-inducible promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT643

    Plasmid

    #8610

    Depositor

    Robert Hartley

    Article

    Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

    Insert

    barstar A
    

    Expression

    Bacterial

    Mutation

    Two cysteines of barstar changed to alanine, C40A…

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPAP001

    Plasmid

    #153488

    Purpose

    Level 1 episomal expression plasmid for P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD

    Plasmid

    #226282

    Purpose

    Tetracycline-inducible expression of Isl1 and Lhx3 for direct reprogramming of canine fibroblasts to induced-motor neurons

    Depositor

    Susannah Sample

    Article

    Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

    Insert

    ISL1:T2A:LHX3 (ISL1, LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Isl1 and Lhx3 linked via T2A

    Expression

    Mammalian

    Available Since

    Nov. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-UbC-rtTA-Ngn2_2A_Sox11-PU​RO

    Plasmid

    #226284

    Purpose

    Tetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neurons

    Depositor

    Susannah Sample

    Article

    Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

    Insert

    NGN2:T2A:SOX11 (SOX11, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    NGN2 and SOX11 linked via T2A

    Expression

    Mammalian

    Available Since

    Nov. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid

    #241142

    Purpose

    Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    tdMCP
    tdPCP
    

    Tags

    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Calmodulin1 (CaM)

    Plasmid

    #240202

    Purpose

    Calmodulin-1 for CaMK2-alpha sensor assays in mammalian cell lines

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    calmodulin-1 (Calm1 Mouse)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    July 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge