163,584 results

1. pmCherry-C1-Nir1-LNS2

   Plasmid

   #220084

   Purpose

   high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   mCherry-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER dCas9-TET1CD

   Plasmid

   #101921

   Purpose

   The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV::SypHy A4

   Plasmid

   #24478

   Depositor

   Leon Lagnado

   Article

   Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

   Insert

   SypHy (Syp Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN-sacB

   Plasmid

   #196249

   Purpose

   Plasmid containing the sacB gene

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attR2-sacB-attL3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sacB promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-TrxRFP2

   Plasmid

   #172322

   Purpose

   an improved fluorescent biosensor for thioredoxin redox

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   TrxRFP2, an improved version from TrxRFP1
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1661-sgMUC4-E3(F+E)

   Plasmid

   #51025

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   optimized sgRNA (MUC4 Human, Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS3Clux

   Plasmid

   #55172

   Purpose

   lux-reporter vector, integration at sacA, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-puro-SNAP-FLAG-CD59

   Plasmid

   #50374

   Purpose

   Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   SNAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b His-p97

    Plasmid

    #169020

    Purpose

    Expresses His-p97 in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-GFP-MT2A

    Plasmid

    #11613

    Depositor

    Steven Johnson

    Insert

    metallothionein 2A (MT2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-GFP

    Plasmid

    #181906

    Purpose

    Fluorescently tagged dead Cas9

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-pHluorin_M153R-CD63-mScarlet

    Plasmid

    #172118

    Purpose

    Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet and pHluorin_M153R in a small extracellu…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS8c_ddCpf1_final

    Plasmid

    #212040

    Purpose

    Bacterial expression of the CRISPRi system for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    ddCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    L-arabinose-inducible promoter PBAD

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-Halo

    Plasmid

    #200197

    Purpose

    Expression for SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-NPC1

    Plasmid

    #132295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMRS-BG42-GFP

    Plasmid

    #220202

    Purpose

    mini-Tn7 GFP expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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