4,170 results

1. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-32a(+)-TGFB3

   Plasmid

   #120288

   Purpose

   Produce human recombinant TGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMV306hsp

   Plasmid

   #26155

   Depositor

   Siouxsie Wiles, Brian Robertson

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   hsp60 promoter
   

   Use

   Mycobacteria integrating vector

   Available Since

   Nov. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-CMV-mCherry-T2A-Puro

   Plasmid

   #72264

   Purpose

   Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

   Depositor

   Kazuhiro Oka

   Insert

   mCherry-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-hGal3

   Plasmid

   #73080

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin-3 (LGALS3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

   Plasmid

   #21845

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Expression

   Mammalian

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Active Wnt3A-V5

    Plasmid

    #43810

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT3A (WNT3A Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAdTrack

    Plasmid

    #16404

    Purpose

    Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTP1183

    Plasmid

    #104163

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIRES2-EGFP-p53 WT

    Plasmid

    #49242

    Purpose

    expresses human wt p53 and EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 (TP53 Human)
    

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV-RFP

    Plasmid

    #26001

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-RFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Raptor_2 shRNA

    Plasmid

    #1858

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-DDB1

    Plasmid

    #19918

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-TPNOX

    Plasmid

    #87853

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    TPNOX
    

    Tags

    C-terminal FLAG tag with a linker

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BcatMutS33/S37.T41/S45

    Plasmid

    #24204

    Depositor

    David Rimm

    Insert

    beta catenin (CTNNB1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S33A, S37A, T41A,S45A

    Promoter

    CMV

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge