4,272 results

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pHIV-Luciferase

Plasmid

#21375

Purpose

self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

Depositor

Bryan Welm

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pSIN-PAmCherry-KFERQ-NE

Plasmid

#102365

Purpose

Lentiviral overexpression of PA-mCherry-KFERQ fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
KFERQ peptide

Use

Lentiviral

Tags

NE

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4-p52

Plasmid

#23289

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p52 (NFKB2 Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f

Plasmid

#162373

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP DEST (736-1)

Plasmid

#19732

Purpose

3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi; Gateway destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2

Plasmid

#116734

Purpose

Lentiviral expression of ERBB2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Article

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-RhoA-Q63L

Plasmid

#12964

Depositor

Gary Bokoch

Insert

RhoA constitutively active (RHOA Human)

Tags

Myc

Expression

Mammalian

Mutation

Q63L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-mTagBFP2

Plasmid

#34632

Depositor

Vladislav Verkhusha

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

mTagBFP2

Expression

Bacterial

Mutation

I174A compared to mTagBFP (enhanced photostabilit…

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-Blue FP

Plasmid

#52115

Purpose

a bicistronic IRES-Blue FP containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-Blue FP

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of TagBFP-Rab5 fusion

Depositor

Gia Voeltz

Article

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMA4008

Plasmid

#70109

Purpose

mtDNA destruction/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
Herpes simplex virus UL12.5M185

Tags

none

Expression

Mammalian

Mutation

N-terminal deletion of UL12.5

Promoter

CMV and RSV

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN155(I152L)

Plasmid

#27097

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

VN155(I152L)

Tags

Myc

Expression

Mammalian

Mutation

I152L

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV OMM-FKBP-V5-sTurboID (N)

Plasmid

#153006

Purpose

Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

OMM (Tom20)-sTurboID (N) (L73)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22C

Plasmid

#91135

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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