19,178 results

1. pSpliceExpress

   Plasmid

   #32485

   Depositor

   Stefan Stamm

   Article

   Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDsRed Monomer C1-YAP2

   Plasmid

   #19057

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Jul 17. ():.

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Red-FPP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C2-NLRP3

   Plasmid

   #73955

   Purpose

   Express EGFP-tagged human NLRP3 in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

   Insert

   NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV iE

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP VAMP7 (1-220)

   Plasmid

   #42316

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 (1-220) (VAMP7 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLH01

   Plasmid

   #117261

   Purpose

   Genome editing for Lactobacillus plantarum WCSF1

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   PorfX-Lp_0640-42, sapK-sappR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. YAP/TAZ-TRE-mStrawberry reporter

   Plasmid

   #158682

   Purpose

   This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Inducible Caspex expression

   Plasmid

   #97421

   Purpose

   For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

   Depositor

   Samuel Myers, Steven Carr

   Article

   Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

   Inserts

   Caspex
   T2A-Gfp
   

   Use

   CRISPR

   Tags

   3xFLAG and V5

   Expression

   Mammalian

   Mutation

   dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

   Promoter

   TRE3G

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-tdTomato

   Plasmid

   #83029

   Purpose

   expresses tdTomato fluorescent protein under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2/v5-DEST hGlut4

    Plasmid

    #18087

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut4 (SLC2A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. STING-V1

    Plasmid

    #124262

    Purpose

    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SF-SON IRES Tomato

    Plasmid

    #214403

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMD2.G-VSV-G-mut

    Plasmid

    #196508

    Purpose

    VSV-G mutant (K47Q, R354A)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    VSV-G
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP_CD47_SU

    Plasmid

    #65473

    Purpose

    GFP tagged CD47 with its own short 3'UTR

    Depositor

    Christine Mayr

    Article

    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

    Inserts

    Signal peptide of CD47
    GFP
    CD47 coding region continued and short 3’UTR.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    F74G

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG423GPD-ccdB

    Plasmid

    #14150

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. BCL2L1_pLX307

    Plasmid

    #98323

    Purpose

    Lentiviral expression of BCL2L1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA PKB T308D S473D pcDNA3

    Plasmid

    #14751

    Depositor

    Jim Woodgett

    Article

    Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

    Insert

    PKB DD (AKT1 Bovine)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T308D S473D. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pCX420

    Plasmid

    #229211

    Purpose

    Cytoplasmic-targeting Csm complex plasmid

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCW57.1 N-term GFP tTA

    Plasmid

    #107551

    Purpose

    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP (optional)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

    Plasmid

    #182306

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NFIA (NFIA Human)
    SOX9 (SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-Bi-directional

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. YIp204-PADH1-atTIR1-9myc

    Plasmid

    #99532

    Purpose

    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    9xMyc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pFX-mCherry-EEA1

    Plasmid

    #174452

    Purpose

    Express mCherry with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with early endosome-targeting sequence
    

    Use

    Tags

    early endosomal antigen 1 (EEA1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC_N_MED13

    Plasmid

    #111689

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    MED13 (MED13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-TLR2-YFP

    Plasmid

    #13016

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge