162,682 results

1. qTAG-N-Blast-dTAG-LMNB1

   Plasmid

   #207776

   Purpose

   Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLexM-SLC7A11

   Plasmid

   #197993

   Purpose

   Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CD28-mCherry

   Plasmid

   #223593

   Purpose

   Lentiviral expression of human CD28 with mCherry tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 35S:RUBY

   Plasmid

   #160908

   Purpose

   RUBY under the control of CaMV 35S promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and CaMV 35S promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2B-GFP-Aurora B WT

   Plasmid

   #184042

   Purpose

   Expresses H2B-GFP fused to Aurora B WT

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Use

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-mScarlet-Puro

   Plasmid

   #207722

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSW002-Pc-mTurquoise2

   Plasmid

   #108235

   Purpose

   Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   Pc promoter - mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for experession in P. fluorescens

   Promoter

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0041-RanCas13b crRNA backbone

   Plasmid

   #103852

   Purpose

   For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-RanCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAIP1

   Plasmid

   #233685

   Purpose

   Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

   Depositor

   Christopher Bahl

   Insert

   Ulp1-like SUMO protease
   

   Use

   Tags

   CAHS-107838 and HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. DRD1-Tango

    Plasmid

    #66268

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    DRD1 (DRD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEV-G2-Km ymNeongreen

    Plasmid

    #193959

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-UltraCas12a-6xNLS

    Plasmid

    #209023

    Purpose

    Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    UltraCas12a-6xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    M537R/F870L

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAKgfp1

    Plasmid

    #14076

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pBabe-c-myc-zeo

    Plasmid

    #17758

    Depositor

    Susan Lindquist

    Article

    Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-WASH1-C-18

    Plasmid

    #55162

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    WASH1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBudORF2-CH

    Plasmid

    #51289

    Purpose

    expresses the codon optimized ORF2 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 ORF2 (L1RE1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti6.2_mTurquoise2

    Plasmid

    #113723

    Purpose

    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO-FOXM1-shRNA-81

    Plasmid

    #89495

    Purpose

    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRSin_1G4 TCRα

    Plasmid

    #187572

    Purpose

    Lentiviral expression TCR alpha(1G4)

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    1G4 TCR alpha (TRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHRSin_1G4 hTCRβ-SNAP

    Plasmid

    #187573

    Purpose

    Lentiviral expression of hTCR beta(1G4), and SNAP

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    1G4 hTCR beta, and SNAP tag (TRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge