20,110 results

Showing: 1981 - 2010 of 20110 results

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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p38-KTR-mStrawberry reporter

Plasmid

#158687

Purpose

This plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023

Plasmid

#90324

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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BirA His-tag pLEMO

Plasmid

#119817

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Tags

6-Histidine-tag

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE NFkB-TA-LUC-UBC-GFP-W

Plasmid

#49343

Purpose

pHAGE NFkB-TA-LUC-UBC-GFP-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

eGFP (eGFP )
Luciferase
NFkB consensus binding sequence
TA
UBC

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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SBE4-Luc

Plasmid

#16495

Purpose

Luciferase reporter containing four copies of the Smad binding site

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad binding element

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

Plasmid

#188778

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-NLS

Plasmid

#168302

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X

Plasmid

#116730

Purpose

Lentiviral expression of DDX3X

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.8TM.BB.3z

Plasmid

#200671

Purpose

Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR2

Plasmid

#116741

Purpose

Lentiviral expression of FGFR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR2 (FGFR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-mCherry

Plasmid

#114472

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

hSyn

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Klf5

Plasmid

#50787

Purpose

retroviral expression of mouse Klf5

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf5 (Klf5 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-Golgi-7

Plasmid

#55052

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212829

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-Keap1

Plasmid

#28023

Depositor

Qing Zhong

Article

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

Keap1 (KEAP1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Showing: 1981 - 2010 of 20110 results

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20,110 results