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We narrowed to 14,052 results for: CRISPR-Cas9

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Showing: 1321 - 1340 of 14052 results

  3. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  5. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  6. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  12. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  13. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Article
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Available Since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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Showing: 1321 - 1340 of 14052 results